GE29-0491-04

HiTrap^® MabSelect SuRe^™

Cytiva 29-0491-04, pack of 1 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• ligand: alkali-stabilized protein A-derived (E. coli)
• 包裝: pack of 1 mL
• 製造商／商標名: Cytiva 29-0491-04
• 存貨情形: not available in North America
• 儲存條件: (20% Ethanol); (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
• 參數: 42 psi
• 床尺寸: 7 mm × 25 mm
• 床體積: 1 mL
• 柱內徑: 7 mm
• 基質: highly cross-linked agarose
• 樣品平均尺寸: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• 工作範圍: 3-12
• 容量: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

描述

一般說明

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids