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GE17-0404-01

HiTrap® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

同義詞:

Protein purification column

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

包裝

pack of 5 × 1 mL

製造商／商標名

Cytiva 17-0404-01

儲存條件

(20% Ehtanol)
 (20% Ethanol)

參數

<4 mL/min flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

尺寸

樣品平均尺寸

34 μm

cleaning

2-9

工作範圍

3-9

容量

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

儲存溫度

2-8°C

相關類別

一般說明

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Lin-Ping Wu et al.
Nature communications, 12(1), 4858-4858 (2021-08-13)
Complement is an enzymatic humoral pattern-recognition defence system of the body. Non-specific deposition of blood biomolecules on nanomedicines triggers complement activation through the alternative pathway, but complement-triggering mechanisms of nanomaterials with dimensions comparable to or smaller than many globular blood

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Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

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General instructions for affinity purification using HiTrap columns from Cytiva.

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Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from GE Healthcare.

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