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重要文件

G2921

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌

BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)

同義詞:

G-6-P-DH

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204

重組細胞

expressed in E. coli

產品線

BioUltra

化驗

≥95% (SDS-PAGE)

形狀

ammonium sulfate suspension

比活性

≥550 units/mg protein (biuret)

異物活動

Creatine Phosphokinase, Phosphoglucose isomerase, lactic dehydrogenase, glutathione reductase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, hexokinase ≤0.001%
Myokinase ≤0.005%

儲存溫度

2-8°C

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一般說明

葡萄糖-6-磷酸脱氢酶(G6PD)是一种X连锁的必需酶,可保护细胞免受氧化应激损伤,特别是红细胞。

應用

葡萄糖-6-磷酸脱氢酶被用于研究热纤梭菌的非典型糖酵解。

生化/生理作用

葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯,作为磷酸戊糖途径的第一步。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步。

單位定義

一个酶活性单位是指在pH 7.8、30°C 下,在 NAD 存在下,每分钟将 1.0 μmol D-6-磷酸葡萄糖氧化为6-磷酸-D-葡萄糖酸盐所需的酶量

外觀

溶于3.2M硫酸铵,含50 mM Tris和1 mM氯化镁,pH 7.5

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Signs of endotracheal intubation in a field setting.
R D Stewart et al.
Annals of emergency medicine, 14(3), 276-277 (1985-03-01)
Jilai Zhou et al.
Applied and environmental microbiology, 79(9), 3000-3008 (2013-02-26)
Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
Myriam Ferrara et al.
Extremophiles : life under extreme conditions, 17(1), 53-62 (2012-11-03)
Psychrophilic organisms have evolved a number of modifications of cellular structures to survive in the cold environment; among them it is worth noting an increased efficiency of enzymes at lower temperatures. Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was purified and characterised
Clara Slade Oliveira et al.
Reproduction (Cambridge, England), 145(1), 9-17 (2012-10-30)
During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation.

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