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G6378

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于面包酵母(酿酒酵母

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

同義詞:

6-磷酸葡糖脱氢酶, G-6-P-DH

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type XV

品質等級

形狀

lyophilized powder

比活性

200-400 units/mg protein (modified Warburg-Christian)

分子量

128 kDa

純化經由

crystallization

應用

diagnostic assay manufacturing

運輸包裝

dry ice

儲存溫度

−20°C

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相關類別

一般說明

葡萄糖6-磷酸脱氢酶(G-6-P-DH)是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是分子量为128kDa(凝胶过滤)的糖蛋白

應用

葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。

生化/生理作用

葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步<<<17>>>。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步。

單位定義

一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下,每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。

外觀

冻干粉,基本上无硫酸盐,按干重计含有大约20% 的柠檬酸钠

準備報告

由 G7877 制备

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Sean Rogers et al.
eLife, 10 (2021-04-08)
Eukaryotes compartmentalize metabolic pathways into sub-cellular domains, but the role of inter-organelle contacts in organizing metabolic reactions remains poorly understood. Here, we show that in response to acute glucose restriction (AGR) yeast undergo metabolic remodeling of their mevalonate pathway that

條款

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

在测定6-磷酸葡萄糖脱氢酶活性时,使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

相關內容

This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.

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