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E3403

Millipore

EZview 红色蛋白 G 亲和凝胶

同義詞:

EZview Affinity Gel, Protein G Affinity Gel

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.56
暫時無法取得訂價和供貨情況

形狀

liquid

分析物化學類別

proteins (Immunoglobulins of various mammalian species)

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

≥8 mg/mL, gel binding capacity (rabbit IgG)

儲存溫度

−20°C

一般說明

EZ view Red蛋白G亲和凝胶通过共价键与4%琼脂糖珠结合。该亲和凝胶用于将抗体IgG的FC部分与样品结合,如细胞裂解物和组织,纯化标记蛋白以进行免疫沉淀分析。红色染料增强了可视性,以获得更有效的结果。

應用

在小规模亲和捕获中,如免疫沉淀,难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂,会导致结果产生数量差异。EZview 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同,但更易于区分沉淀和上清液。 这将获得更准确的数据,因为蛋白质损失减少。

特點和優勢

  • 可见性增加-红色可降低意外吸入的风险
  • 通过减少意外损失提高目标蛋白的回收率
  • 重现性更高-收率更一致

外觀

1:1(v/v)混悬液溶于含有50%甘油和15 ppm Kathon的PBS中。
PBS中50%悬浮液加入50%甘油及0.0015%

法律資訊

EZview is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Yuval Malka et al.
Nature communications, 8(1), 2029-2029 (2017-12-13)
The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and
Min Mo et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(46), 19527-19532 (2009-11-06)
The anaphase-promoting complex (APC), or cyclosome, is a ubiquitin ligase with major roles in cell cycle regulation. It is required for mitotic exit, but must be deactivated for the G(1)/S phase transition to occur. APC consists of at least 12
Álvaro Quintanal-Villalonga et al.
EBioMedicine, 53, 102683-102683 (2020-03-03)
Fibroblast growth factor receptor (FGFR)1 and FGFR4 have been associated with tumorigenesis in a variety of tumour types. As a therapeutic approach, their inhibition has been attempted in different types of malignancies, including lung cancer, and was initially focused on
Juri Kazakevych et al.
Nucleic acids research, 45(10), 5770-5784 (2017-03-24)
Epigenetic mechanisms, including chromatin structure, chromatin dynamics and histone modifications play an important role for maintenance and differentiation of pluripotent embryonic stem cells. However, little is known about the molecular mechanisms of adult stem cell specification and differentiation. Here, we
Alvaro Quintanal-Villalonga et al.
Lung cancer (Amsterdam, Netherlands), 131, 112-121 (2019-04-28)
Lung adenocarcinoma accounts for approximately half of lung cancer cases. Twenty to 50% of tumors of this type harbor mutations affecting epidermal growth factor receptor (EGFR) expression or activity, which can be therapeutically targeted. EGFR inhibitors in this context exhibit

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