This product is prepared in 50 mM sodium acetate buffer containing 1 mM EDTA, pH 5.0. This buffer may also be used to dilute the material further. This item has not been tested with other buffers as a diluent, however 25 mM MES has also been cited in the literature. See the link below to review this reference:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cathepsin-b
推薦產品
生物源
human liver
品質等級
描述
Merck USA index - 14, 1905
化驗
>95% (SDS-PAGE)
形狀
liquid
比活性
≥50 units/mg protein
純化經由
affinity chromatography
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
技術
activity assay: suitable
pH值範圍
3.5—6.0
適合性
suitable for molecular biology
應用
life science and biopharma
運輸包裝
wet ice
儲存溫度
−70°C
基因資訊
human ... CTSB(1508)
一般說明
Research area: Cell Signaling
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase[1] and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[2]
Optimal pH: 3.5-6.0.
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase[1] and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[2]
Optimal pH: 3.5-6.0.
應用
生化／生理作用
Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins.[2] Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
單位定義
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.
外觀
In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
準備報告
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
重構
Following initial thaw, aliquot and freeze (-70°C).
其他說明
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
BinQing Wei et al.
Journal of medicinal chemistry, 61(3), 989-1000 (2017-12-12)
Antibody-drug conjugates (ADCs) have become an important therapeutic modality for oncology, with three approved by the FDA and over 60 others in clinical trials. Despite the progress, improvements in ADC therapeutic index are desired. Peptide-based ADC linkers that are cleaved
Lysosomal pathways to cell death and their therapeutic applications
Cesen MH, et al.
Experimental Cell Research, 1245-1251 (2012)
Lorna Kategaya et al.
Nature, 550(7677), 534-538 (2017-10-19)
The ubiquitin system regulates essential cellular processes in eukaryotes. Ubiquitin is ligated to substrate proteins as monomers or chains and the topology of ubiquitin modifications regulates substrate interactions with specific proteins. Thus ubiquitination directs a variety of substrate fates including
Vito Turk et al.
Biochimica et biophysica acta, 1824(1), 68-88 (2011-10-26)
It is more than 50 years since the lysosome was discovered. Since then its hydrolytic machinery, including proteases and other hydrolases, has been fairly well identified and characterized. Among these are the cysteine cathepsins, members of the family of papain-like
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We have bought this product (Cathepsin B, Human Liver, 219364), and the size is 5U. We are facing a problem with the working stock concentration. Do we need to resubstitute the volume into MQ water or buffer?
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