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71206

Millipore

Benzonase® Nuclease HC

Purity > 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

同義詞:

内切核酸酶 来源于粘质沙雷氏菌

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71206-3CN


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71206-3CN

About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.54


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產品名稱

Benzonase® 核酸酶HC,纯度> 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

生物源

Serratia marcescens

品質等級

重組細胞

expressed in E. coli

化驗

>99% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

製造商/商標名

Novagen®

儲存條件

OK to freeze

濃度

≥250 units/μL

雜質

<0.25 EU/kU Total endotoxin

應用

research use

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

Benzonase® 核酸酶是一种用基因工程方法从粘质沙雷氏菌获得的核酸内切酶。它降解所有形式的DNA和RNA(单链、双链、线性和环状),而没有蛋白水解活性。它能够在较大范围的条件下工作,并且具有极高的比活度。该酶将核酸完全消化为长度为2至5个碱基(低于杂交极限)的5′-一磷酸酯化的寡核苷酸,非常适合从重组蛋白中去除核酸,从而符合FDA关于核酸污染的指南。Benzonase迅速水解核酸的能力使该酶成为降低粘度、减少加工时间并提高蛋白质产量的绝佳选择。例如,该酶与 BugBuster 和 PopCulture 蛋白质提取试剂兼容,因此可以与这些试剂一起添加,以消除大肠杆菌提取物中的粘度并去除核酸。

该酶包括两个 30kDa 的亚基。它在pH值6-10之间,温度0-42°C 起作用,并需要1-2mM Mg2+激活。该酶在离子和非离子型洗涤剂、还原剂、PMSF(1 mM)、EDTA(1 mM)和尿素(相对活性取决于特定条件)的存在下也具有活性。>150 mM单价阳离子,>100 mM磷酸盐,>100 mM硫酸铵或>100 mM盐酸胍能抑制其活性。

可提供超纯Benzonase 核酸酶(>99%,SDS-PAGE)和纯Benzonase核酸酶(>90%),标准浓度为25-29 U/µl,高浓度(HC)为250U/lµ。两种制剂均不含可检测的蛋白酶,并且比活度> 1 × 106蛋白。纯度>99%的产品经过内毒素测试,并含有<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >99%(SDS-PAGE)。

應用

用于去除蛋白样品中的核酸。

生化/生理作用

消化天然或热变性的DNA和RNA。

警告

毒性:标准处理(A)

單位定義

一个单位定义为在 30 分钟内导致ΔA₂₆₀为1.0的酶量,这对应于37 µg DNA的完全消化。

法律資訊

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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Rajeevkumar Raveendran Nair et al.
iScience, 23(3), 100888-100888 (2020-02-23)
Although a variety of remarkable molecular tools for studying neural circuits have recently been developed, the ability to deploy them in particular neuronal subtypes is limited by the fact that native promoters are almost never specific enough. We recently showed
William C Buchta et al.
Molecular neurobiology, 57(1), 346-357 (2019-07-31)
Cocaine addiction remains a major health concern with limited effective treatment options. A better understanding of mechanisms underlying relapse may help inform the development of new pharmacotherapies. Emerging evidence suggests that collapsin response mediator protein 2 (CRMP2) regulates presynaptic excitatory
Rodrigo Villaseñor et al.
Nature biotechnology, 38(6), 728-736 (2020-03-04)
Chromatin modifications regulate genome function by recruiting proteins to the genome. However, the protein composition at distinct chromatin modifications has yet to be fully characterized. In this study, we used natural protein domains as modular building blocks to develop engineered
Alexandros P Drainas et al.
Cell reports, 31(1), 107465-107465 (2020-04-09)
TP53 deficiency is the most common alteration in cancer; however, this alone is typically insufficient to drive tumorigenesis. To identify genes promoting tumorigenesis in combination with TP53 deficiency, we perform genome-wide CRISPR-Cas9 knockout screens coupled with proliferation and transformation assays
Annabel Quinet et al.
Molecular cell, 77(3), 461-474 (2019-11-05)
Acute treatment with replication-stalling chemotherapeutics causes reversal of replication forks. BRCA proteins protect reversed forks from nucleolytic degradation, and their loss leads to chemosensitivity. Here, we show that fork degradation is no longer detectable in BRCA1-deficient cancer cells exposed to

文章

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

本頁面列出 9 個關於 Benzonase® 核酸酶的常見問題與解答。

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

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