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重要文件

D7777

Sigma-Aldrich

杜氏改良 Eagle 培养基 - 高葡萄糖

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

運輸包裝

ambient

儲存溫度

2-8°C

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應用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

數量

按照每升培养基13.5克粉末配制。

重構

补充 3.7g/L 碳酸氢钠

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1


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粉狀介質和鹽混合物具有極強的吸濕性,因此應防止大氣中的濕氣進入。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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