推薦產品
product name
杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
形狀
powder
技術
cell culture | mammalian: suitable
成分
phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no
運輸包裝
ambient
儲存溫度
2-8°C
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應用
Dulbecco′s 改良Eagles 培养基- 高葡萄糖已被用于各种细胞类型的培养基，包括人宫颈内上皮End1/E6E7细胞、人腺癌上皮细胞（HeLa）、人肺泡腺癌上皮细胞（A549）、人骨肉瘤上皮细胞、人二倍体细胞系、原代肝细胞和滑膜组织
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
數量
按照每升培养基13.4克粉末配制。
重構
补充3.7g/L碳酸氢钠。
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產品號碼
描述
訂價
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
Cell culture protocol for passaging and splitting suspension cell lines
傳代和分裂懸浮細胞系的細胞培養方案
條款
粉狀介質和鹽混合物具有極強的吸濕性，因此應防止大氣中的濕氣進入。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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