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D8764

Sigma-Aldrich

脱氧核糖核酸酶II 来源于牛脾脏

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

同義詞:

DNase II, 脱氧核苷酸酶 3′-寡核苷酸脱氢酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
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生物源

bovine spleen

種類

Type V

形狀

essentially salt-free, lyophilized powder

比活性

≥1,000 units/mg protein

分子量

38 kDa

濃度

≥50% protein

技術

DNA extraction: suitable
tissue culture: suitable

適合性

suitable for molecular biology

應用

cell analysis

異物活動

RNase ≤0.1%

儲存溫度

−20°C

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應用

牛脾脱氧核糖核酸酶Ⅱ已用于:
  • 作为从结肠样品分离免疫细胞的消化混合液组分[1]
  • 消化视网膜外植体以制备单细胞悬液[2]
  • 分离人结肠组织的基质细胞[3]
  • 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分[4]

生化/生理作用

脱氧核糖核酸酶II,也称为酸性DNA酶,可水解天然和变性DNA中的脱氧核糖核苷酸连接键,产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。[5]

單位定義

在 pH 4.6 25℃条件下,一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260;[Mg2+] = 0.83 mM

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.

Questions

  1. What is the best buffer to dissolve the lyophilized powder in? What is the best final concentration to be prepared?

    1 answer
    1. According to the Enzymatic Assay Test Procedure, a solution should be prepared immediately before use, containing 500–1000 Kunitz units/ml in cold deionized water. More information can be found in the document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/769/284/d8764enz.pdf

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