Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
推薦產品
一般說明
SIGMA FAST™ DAB片剂（含金属增强剂）可直接制备用于过氧化物酶活性检测的活性底物溶液，无需额外的缓冲液或步骤。 常用作免疫组织学的沉淀底物。
應用
通过添加CoCl2可以增强DAB反应，并产生更强的耐酒精色斑。
重構
将每个片剂组合溶于5 mL去离子水中，得到含有DAB和金属强化剂的即用型缓冲溶液。
其他說明
法律資訊
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
條款
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
使用本方案，可在石蠟包埋組織切片中透過染色和顯現特定抗原，進行整個免疫組織化學 (IHC) 程序。
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How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 answer-
Helpful?
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What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
1 answer-
This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 answer-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
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