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D6815

Sigma-Aldrich

3,3′-二氨基联苯胺 (DAB) 增强型液体底物系统 四盐酸盐

peroxidase substrate, liquid, suitable for membrane applications

同義詞:

DAB membrane substrate

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About This Item

MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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產品名稱

3,3′-二氨基联苯胺 (DAB) 增强型液体底物系统 四盐酸盐, for Membrane Applications

品質等級

形狀

liquid

運輸包裝

wet ice

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI 密鑰

KJDSORYAHBAGPP-UHFFFAOYSA-N

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應用

DAB(3,3′-二氨基联苯胺四盐酸盐)双组分增强液底物系统是过氧化物酶的沉淀底物系统。DAB 双组分液体底物系统会产生棕色沉淀,可用于免疫组织学和免疫印迹染色程序。
3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
  • in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining[1]
  • in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi[2]
  • in immunoperoxidase staining of lung tissue[3]

生化/生理作用

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.[4]

包裝

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

注意

在 2-8℃ 下储存于原容器中时可稳定储存长达 18 个月。

數量

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Carc. 1B - Muta. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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存取文件庫

Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49(1), 8-8 (2012)
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106(3), 343-354 (2011)
Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49, 8-8 null
Nanette Mittereder et al.
Journal of immunology (Baltimore, Md. : 1950), 197(2), 491-503 (2016-06-15)
ICOS, a member of the CD28 family, represents a key molecule that regulates adaptive responses to foreign Ags. ICOS is prominently expressed on T follicular helper (TFH) cells, a specialized CD4(+) T cell subset that orchestrates B cell differentiation within
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106, 343-354 (2011)

文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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