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重要文件

D4293

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST, chromogenic, tablet

同義詞:

DAB tablets

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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產品名稱

SIGMAFAST 3,3′-二氨基联苯胺片, tablet, To prepare 5 mL

品質等級

形狀

tablet

儲存溫度

−20°C

SMILES 字串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI 密鑰

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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應用

σ 快速 3,3′-二氨基联苯胺片已被用作显色剂,用于性腺组织、[1] 胸腺、[2] 牙齿慢性根尖周病变 [3] 和胆囊切片的免疫组织化学显色。[4]
开发用于免疫组织学和免疫印迹,作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物,因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水,封片于树脂介质中,并储存以备将来引用。SIGMAFAST DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中

重構

将每片片剂溶于 5 mL 去离子水中,得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

1–2 of 2 Questions  
  1. Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?

    1 answer
    1. This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.

      Helpful?

  2. Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda

    1 answer
    1. D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7

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