推薦產品
特異性
4-氯-1-萘酚是一种适合用于免疫印迹程序的过氧化物酶底物。该底物产生的不溶性最终产物为蓝色,可目视观察。
應用
4-氯-1-萘酚已被用作酶联免疫斑点(ELISpot)试验的底物,用于检测斑点。它还被用作沉淀生色底物,用于免疫印迹后抗原−抗体反应性条带的成像。
數量
每片含 30 mg 基质。
重構
将 1 片片剂溶于 10 mL 甲醇中。向 10 mL 三乙醇胺缓冲盐水 (pH 7.5) 中加入 2 mL 甲醇贮备液。临用前加入 5μl 新鲜 30% 过氧化氢(Sigma 产品编号:H-1009)。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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文章
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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