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B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

同義詞:

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83
暫時無法取得訂價和供貨情況

產品名稱

用于膜的BCIP®/NBT-紫色液体底物系统, ready to use alkaline phosphatase substrate

品質等級

100

形狀

liquid

儲存溫度

2-8°C

相關類別

應用

BCIP®/NBT-紫色液体膜底物系统已被用作碱性磷酸酶的底物
  • 用于通过免疫印迹法检测二抗[1]
  • 用于ELISPOT测定分析[2]
  • 用于斑点印迹分析[3]
  • 用于免疫印迹分析，以可视化硝酸纤维素膜上的蛋白条带[4]

生化／生理作用

5-溴-4-氯-3-吲哚基磷酸酯 (BCIP)/硝基蓝四唑（NBT）/硝基蓝四唑鎓(NBT)是用于检测碱性磷酸酶(AP)的显色底物系统。AP水解BCIP形成中间体无色靛蓝化合物，并进一步被NBT氧化形成5,5′-二溴-4,4′-二氯靛蓝(BCI)。其中，NBT被还原为不溶的紫色双甲双胍(DF)，形成NBT-DF。NBT-DF和BCI的混合物形成深紫色沉淀。AP可水解多种BCIP分子，因此成为了一种高灵敏度方法。[4]

注意

在2-8 °C稳定至少一年。

外觀

即用型。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H317

危險分類

Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCH2415
MKCH2414
MKCH2413
MKCF9800
MKCF9801

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Xudong Wang et al.
Frontiers in cell and developmental biology, 9, 690502-690502 (2021-08-28)
Melatonin (Mlt) confers potential antitumor effects in various types of cancer. However, to the best of our knowledge, the role of Mlt in the giant cell tumor of bone (GCTB) remains unknown. Moreover, further research is required to assess whether
pSTAT5 and ERK exhibit different expression in myeloproliferative neoplasms
Wisniewska-Chudy E, et al.
Oncology Reports, 37(4), 2295-2307 (2017)
T W J M Van Herpen et al.
Annals of botany, 102(3), 331-342 (2008-07-16)
Alpha-gliadin proteins are important for the industrial quality of bread wheat flour, but they also contain many epitopes that can trigger celiac (coeliac) disease (CD). The B-genome-encoded alpha-gliadin genes, however, contain very few epitopes. Controlling alpha-gliadin gene expression in wheat
Characterization of the 41 kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor
Shi C and Miller JD
Molecular Immunology, 48(15-16), 1827-1834 (2011)
Membrane bioreactor separator system for integrated IgG fragmentation and Fab purification
Yu D and Ghosh R
Journal of Immunological Methods, 359(1-2), 37-41 (2010)
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文章

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

相關內容

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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