推薦產品
一般說明
反应产物呈蓝色，不溶于水。蓝色为肉眼可见。
配方：NBT：C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
评估: 肉眼
配方：NBT：C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
评估: 肉眼
應用
- 该溶液用于通过印迹方案灵敏检测碱性磷酸酶(AP)，适用于：DNA印迹
- RNA印迹
- 蛋白质印迹
- 菌落及菌斑清除
- 原位杂交
- 以及免疫组织化学和免疫化学应用
外觀
溶液中含硝基蓝四唑氯铵18.75 mg/ml, 5-溴-4-氯-3-吲哚基磷酸盐9.4 mg/ml，甲苯胺盐67% (DMSO) (v/v)。
注: 溶液颜色可能从黄色到淡棕色不等。
注: 溶液颜色可能从黄色到淡棕色不等。
準備報告
工作溶液：制备染色液
该溶液需临使用前配制。
该溶液需临使用前配制。
- 除DIG体系外的其他应用：向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2中加入200 μl的储备溶液。
- DIG体系应用：向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl中加入200 μl的储备溶液
其他說明
仅用于生命科学研究。不可用于诊断。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
does not flash
閃點（°C）
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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條款
NBT/BCIP 溶液是在 Southern 印迹、Northern 印迹和 Western 印迹等印迹方案中检测碱性磷酸酶 (AP) 的理想选择。NBT/BCIP 與硝化纖維膜及尼龍膜相容。
NBT/BCIP Stock Solution Protocol & Troubleshooting
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