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Merck
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A7345

Millipore

抗-V5 琼脂糖亲和凝胶 小鼠抗

purified immunoglobulin, clone V5-10

同義詞:

单克隆抗-V5

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56
暫時無法取得訂價和供貨情況

生物源

mouse

共軛

agarose conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

V5-10, monoclonal

分析物化學類別

proteins (V5)

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

2.5 nmol/mL, gel (V5 fusion protein)

運輸包裝

wet ice

儲存溫度

2-8°C

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一般說明

抗-V5 琼脂糖亲和凝胶由与溴化氰活化的珠状琼脂糖偶联的单克隆抗-V5 抗体组成。 该抗体识别转染的哺乳动物细胞中表达的 V5 标记的重组融合蛋白。
抗-V5琼脂糖亲和凝胶由纯化的单克隆抗-V5抗体免疫球蛋白部分与溴化氰活化的珠状琼脂糖偶联组成,单克隆抗-V5抗体从 V5-10杂交瘤腹水中分离。纯化的单克隆抗体固定在琼脂糖上。

特異性

识别 V5 标签(GKPIPNPLLGLDST)融合蛋白

應用

鼠源抗V5琼脂糖亲和凝胶抗体用于免疫沉淀[1][2][3]和重组蛋白纯化。 [4][5]

生化/生理作用

重组 DNA 技术可将目的基因附着在特定序列或基因上,提供′亲和柄′设计,选择性识别和纯化目的蛋白。 [6] 在给定基因中添加V5标签序列(GKPIPNPLLGLDST)[6]会产生稳定的融合产物,这一产物似乎不会干扰蛋白的生物活性或干扰标记产物的生物分布。研究人员已经设计出许多重组蛋白表达衍生自猴副流感病毒V5的短序列,这些短序列称为V5-标签。[7]抗“标签”琼脂糖结合物可以促进检测、分离和纯化这些蛋白质。[7]

外觀

将珠状琼脂糖(1:1v / v)悬浮于含有 15mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水中。

儲存和穩定性

为保证连续使用和延长存储时间,请在2°C至8°C条件下存储。请勿冷冻。

免責聲明

除非产品目录中另有说明,否则我们的产品仅供研究使用,不得在人类(或动物)上用于任何其他目的,包括但不限于未经授权的商业使用、体外诊断、离体(或体内)治疗或进行任何类型的摄入或应用。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Lynnette C Goatley et al.
Journal of virology, 85(7), 3294-3305 (2011-01-21)
The African swine fever virus (ASFV)-encoded CD2v transmembrane protein is required for the hemadsorption of red blood cells around infected cells and is also required for the inhibition of bystander lymphocyte proliferation in response to mitogens. We studied the expression
Q Meng et al.
Analytical biochemistry, 421(2), 351-361 (2011-11-01)
Quantitation of individual monoclonal antibodies (mAbs) within a combined antibody drug product is required for preclinical and clinical drug development, including pharmacokinetic (PK), toxicology, stability, and biochemical characterization studies of such drugs. We have developed an antitoxin, XOMA 3AB, consisting
Arko Dasgupta et al.
PLoS genetics, 11(5), e1005215-e1005215 (2015-05-16)
Most organisms on earth sense light through the use of chromophore-bearing photoreceptive proteins with distinct and characteristic photocycle lengths, yet the biological significance of this adduct decay length is neither understood nor has been tested. In the filamentous fungus Neurospora
Babita Madan et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(21), E2945-E2954 (2016-05-11)
The Wnt signaling pathways play pivotal roles in carcinogenesis. Modulation of the cell-surface abundance of Wnt receptors is emerging as an important mechanism for regulating sensitivity to Wnt ligands. Endocytosis and degradation of the Wnt receptors Frizzled (Fzd) and lipoprotein-related
Maki Ohashi et al.
The Journal of biological chemistry, 285(22), 16408-16415 (2010-03-23)
NKG2D is an important activating receptor on lymphocytes. In human, it interacts with two groups of ligands: the major histocompatibility complex class I chain-related A/B (MICA/B) family and the UL-16 binding protein (ULBP) family, also known as retinoic acid early

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