This product may be prepared in 50 mM TES buffer, pH 7.0, with 1 mM MgCl2, at 1 mg/mL. It is also soluble in water or TE buffer. Please see the link below to review the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/382/514/m9901pis-ms.pdf
推薦產品
生物源
bacterial (Streptomyces globisporus)
品質等級
形狀
lyophilized powder
比活性
≥4000 units/mg protein (biuret)
分子量
23 kDa
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成,可能是一种有效的龋病防治剂。 [1]
應用
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
生化/生理作用
变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶,可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。
單位定義
以粪链球菌细胞壁混悬液为底物,在1mL体积中,1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
外觀
冻干粉,含Ficoll® 和琥珀酸钠缓冲盐
其他說明
法律資訊
Ficoll is a registered trademark of Cytiva
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個:
分析證明 (COA)
Lot/Batch Number
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條款
This procedure may be used for Mutanolysin products.
在测定变溶菌素活性时,使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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Hi, Could you advise the best way to resuspend mutanolysin M9901. Will it be soluble in water?
1 answer-
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