The milligram quantity of this product will vary from lot to lot. The vial content is dependent upon the protein content and enzymatic activity. The minimum protein content will be 20% and the minimum activity will be 2000 units per milligram of protein. As an example, a batch that reports a protein content of 20% and an activity of 5000 units per milligram protein will have a vial content of 2 mg for the 10,000 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l2524#product-documentation
推薦產品
生物源
bacterial (Arthrobacter luteus)
品質等級
形狀
lyophilized powder
比活性
≥2,000 units/mg protein
成份
Protein, ≥20% biuret
技術
cell based assay: suitable
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
儲存溫度
−20°C
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應用
藤黄节杆菌(Arthrobacter luteus)溶壁酶用于：
- 制备原生质球
- 作为消化液组分，用于孵育酵母细胞消化细胞壁
- 酶解菌丝体沉淀以制备原生质体
生化／生理作用
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可断链糖基之间的β(1→3)和β(1→4)键。溶菌酶是巨噬细胞介导宿主反应的指示剂，与白细胞死亡有关，具有高周转率。多种慢性炎症、炎症性肠病、血液病和肾病中都发现血清溶菌酶浓度提高。鸡蛋白中的C型溶菌酶常用作蛋白结构和功能的研究模型。胞壁质酶主要对革兰氏阳性菌具有杀菌活性。手册所述凝胶过滤色谱法测定分子量是现有发表方法的改良版。该试剂盒中的蛋白标准品可能适合其他色谱方法，如高效液相色谱(HPLC)。但是，某些缓冲体系可能会影响白蛋白和碳酸酐酶的洗脱体积。该试剂盒中的蛋白分子量范围为29 kDa至699 kDa。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
酵母细胞很难被破坏，因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶（如溶壁酶）诱导部分原生质球形成，从酵母中提取DNA。[4]随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁，并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。[5]溶壁酶适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。[6]
單位定義
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
外觀
部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂
其他說明
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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條款
本程序可用於以 Baker's yeast 為底物的溶酶活性測定。
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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How many milligrams is 10,000 units of Product L2524 Lyticase from Arthrobacter luteus?
1 answer-
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Is it purified downstream by affinity chromatography or is it just an ammonium sulfate precipitate?
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This product is purified by conventional chromatography following the initial ammonium sulfate precipitation process.
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What is the recommended working concentration of L2524, if a typical 10mg/ml concentration of L1412 is used?
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There have been no tests conducted for product L2524 application on the fungus Aspergillus fumigatus. However, according to a citation in Vet Pathol, 2U/100 ul of fungal extraction has shown good results. The citation is titled "Panfungal Polymerase Chain Reaction for Identification of Fungal Pathogens in Formalin-Fixed Animal Tissues" and was published in July 2017. The DOI is 10.1177/0300985817698207, and the PMID is 28346123
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What is the molecular weight of this product?
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The molecular weight of Lyticase has not been determined.
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What is the molecular weight of this product?
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The molecular weight of Lyticase is not determined.
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I see it is suggested to reconstitute Lyticase fresh. However, if that is not possible, can you store reconstituted Lyticase, L2524, at -20C? If so, for how long?
1 answer-
The solution stability of this product has not been determined. However, according to Cold Spring Harbor Protocols, lyticase may be stored up to 6 months at 4 deg. C when prepared in 10 mM Tris-CL containing 20% glycerol. This has not been validated. See the link below to this citation:
https://cshprotocols.cshlp.org/content/2017/2/pdb.rec090381.full?rss=1Helpful?
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How can I store Product L2524, Lyticase, once reconstituted?
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When lyticase is reconstituted and stored, it may be stable for no more than 4-6 hours before serious loss of activity is observed. It is suggested that fresh solutions be prepared for use.
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How do I solubilize Product L2524, Lyticase?
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The quality control enzymatic assay protocol for Lyticase uses cold deionized water to make a solution at 500 units/mL.
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How can Product L2524, Lyticase, be used to make yeast spheroplasts?
1 answer-
The use of lyticase in combination with chitinase and glucuronidase in the preparation of spheroplasts from C. albicans has been described in the following reference: Calvert, C.M., and Sanders, D., "Inositol Trisphosphate-dependent and -independent Ca2+ Mobilization Pathways at the Vacuolar Membrane of Candida albicans". J. Biol. Chem. 270, 7272-7280 (1995).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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