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75746

Sigma-Aldrich

十二烷基硫酸钠

≥99.0% (GC), dust-free pellets

同義詞:

SDS, 十二烷基硫酸盐 钠盐, 十二烷基硫酸钠, 月桂基硫酸盐 钠盐, 月桂基硫酸钠

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About This Item

線性公式:
CH3(CH2)11OSO3Na
CAS號碼:
分子量::
288.38
Beilstein:
3599286
EC號碼:
MDL號碼:
分類程式碼代碼:
12161900
eCl@ss:
39093306
PubChem物質ID:
NACRES:
NA.25
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描述

anionic

品質等級

化驗

≥99.0% (GC)

形狀

rod

分子量

average mol wt 18,000

凝集数

62

損耗

≤5% loss on drying

CMC

7-10 (lit.)

mp

204 - 207  °C (399 - 405 °F)
204-207 °C (lit.)

轉變溫度

cloud point >100 °C(lit.)

溶解度

H2O: soluble 0.29 gm in 10ml, clear, colorless
H2O: 0.1 M at 20 °C, clear, colorless
130 g/L at 20 °C (68 °F)

密度

1 g/cm3

負離子痕跡

chloride (Cl-): ≤200 mg/kg

正離子痕跡

Ca: ≤25 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤10 mg/kg
K: ≤200 mg/kg
Mg: ≤20 mg/kg
Mn: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

40(lit.)

SMILES 字串

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI 密鑰

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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一般說明

十二烷基硫酸钠(SDS)是一种有机钠盐,在生物化学研究中用作洗涤剂和蛋白质变性剂。其独特的结构包括长链脂肪基团和带负电荷的硫酸盐,使其成为使蛋白质变性的有效试剂。这种特性在电泳中特别有价值,电泳是一种用于分离和分析蛋白质等大分子的技术。SDS带负电荷,与蛋白质和脂质相互作用,形成胶束结构,有助于溶解和稳定这些生物分子。

應用

十二烷基硫酸钠(SDS)已用于:
  • 带负电荷的表面活性剂,研究其在表面活性剂稳定乳液的微滤期间对过滤通量和膜污染的影响
  • 植物提取缓冲液和传递缓冲液的组分用于蛋白质印迹
  • 5× Laemmli样品缓冲液(5× LB)和10×SDS运行缓冲液的组分,以进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹

阴离子洗涤剂

生化/生理作用

十二烷基硫酸钠(SDS)通过硫酸基团和烷基链有效地与蛋白质的正电荷和疏水残基结合。在结构生物学和蛋白质折叠/去折叠研究中,它是一种重要的清洁剂。SDS可刺激蛋白质结果中α-螺旋链的产生。它具有促进多种蛋白质,包括牛血清蛋白(BSA)、β-乳球蛋白、α-乳清蛋白和蛋清溶菌酶等,在高浓度下的协同去折叠能力。 

特點和優勢

  • 经测试确认重金属污染水平较低,确保适用于各种应用
  • 检测痕量阴离子和阳离子
  • 生化研究的高纯度产品

其他說明

如需了解生化试剂系列的更多信息,请填写此表

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

338.0 °F

閃點(°C)

170 °C

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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J Kamerbeek et al.
Journal of clinical microbiology, 35(4), 907-914 (1997-04-01)
Widespread use of DNA restriction fragment length polymorphism (RFLP) to differentiate strains of Mycobacterium tuberculosis to monitor the transmission of tuberculosis has been hampered by the need to culture this slow-growing organism and by the level of technical sophistication needed
D Wessel et al.
Analytical biochemistry, 138(1), 141-143 (1984-04-01)
A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the
K Fujimoto
Journal of cell science, 108 ( Pt 11), 3443-3449 (1995-11-01)
We propose a new electron microscopic method, the sodium dodecylsulphate (SDS)-digested freeze-fracture replica labeling technique, to study the two-dimensional distribution of integral membrane proteins in cellular membranes. Unfixed tissue slices were frozen with liquid helium, freeze-fractured, and replicated in a
The gross conformation of protein-sodium dodecyl sulfate complexes.
J A Reynolds et al.
The Journal of biological chemistry, 245(19), 5161-5165 (1970-10-10)
Shane Stegeman et al.
Endocrine-related cancer, 22(2), 265-276 (2015-02-12)
The oncogene MDM4, also known as MDMX or HDMX, contributes to cancer susceptibility and progression through its capacity to negatively regulate a range of genes with tumour-suppressive functions. As part of a recent genome-wide association study it was determined that

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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