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70540

Sigma-Aldrich

萘酚黄 S

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

同義詞:

2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐

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About This Item

經驗公式(希爾表示法):
C10H4N2Na2O8S
CAS號碼:
分子量::
358.19
顏色索引編號:
10316
Beilstein:
3839220
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

for microscopy (Hist.)
for the precipitation (of amino acids and peptides)

形狀

powder

溶解度

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI 密鑰

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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應用

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

生化/生理作用

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

分析報告

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

象形圖

Health hazardExclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1 - STOT RE 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
J Tas et al.
Acta histochemica. Supplementband, 20, 69-73 (1979-01-01)
After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye
W M Frederiks et al.
Histochemistry, 68(1), 49-53 (1980-01-01)
The purpose of this study is to compare the protein content of parenchymal and non-parenchymal nuclei, as isolated from rat liver. The nuclei have been separated by means of a 1 g-sedimentation technique. The protein content of the separated nuclei
S Gutschmidt et al.
Histochemistry, 72(3), 467-479 (1981-01-01)
In order to quantify the amount of protein in the small intestinal brush border region at different villus sites, cryostat sections of adult rat jejunum were stained with Naphthol Yellow S, Dinitrofluorobenzene and Coomassie Brilliant Blue and the dye deposits
F A Bignone et al.
Bollettino della Societa italiana di biologia sperimentale, 57(9), 950-955 (1981-05-15)
In the present work we studied the reversibility of the binding reaction of Naphthol Yellow S to proteins using a Testing Automatic System Leitz. We presently think that the NY-S staining procedure, can be used as a reliable quantitative method

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