The recommended protocol for using Alizarin Red S involves a pH 4.2 method. Prepare the solution by adding 2 grams of Alizarin Red S to 100 ml of deionized water and mix well. Adjust the pH to 4.1 to 4.3 using a 0.5% ammonium hydroxide solution. It is important to use a relatively new bottle of ammonium hydroxide. If the pH is initially more alkaline than 4.2, use HCl to lower it below 4.2, then adjust with ammonium hydroxide. The pH and presence of ammonium ions are critical. Yellow background staining is acceptable, and if there is no calcium in the tissue, the staining will appear yellow. Ensure a positive control is used that has not been exposed to decalcifying agents, as these will remove calcium from tissue sections.
A5533
茜素红 S
certified by the Biological Stain Commission, powder
同義詞:
3,4-二羟基-9,10-二氧代-2-蒽磺酸 钠盐, Sodium alizarinsulfonate, 水溶性茜素红, 茜素 S 钠, 茜素磺酸 钠盐, 茜素磺酸钠, 茜素胭脂红
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About This Item
經驗公式（希爾表示法）:
C14H7NaO7S
CAS號碼:
分子量：:
342.26
顏色索引編號:
58005
Beilstein:
4117090
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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產品名稱
茜素红 S, certified by the Biological Stain Commission
等級
certified by the Biological Stain Commission
品質等級
形狀
powder
濃度
0.015 g/L in 0.1 M NaOH
技術
titration: suitable
顏色
dark red
yellow-orange to dark orange
溶解度
H2O: 1 mg/mL
ε (extinction coefficient)
592-598 nm at 0.015 g/L in 0.1 M NaOH
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
[Na+].Oc1c(O)c(cc2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI
1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1
InChI 密鑰
HFVAFDPGUJEFBQ-UHFFFAOYSA-M
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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My Alizarin red stain is staining my cells yellow instead of red. How should I adjust the solution so that it stains the cells red?
1 answer-
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What is the procedure for preparing a staining solution for Alizarin Red S when using product A5533?
1 answer-
The Alizarin Red S dye is available as a powdered dye, with catalog number A5533 recommended (BSC certified pH 7.0). Dissolve 2 grams of Alizarin Red S in 100 ml of distilled water and adjusting the pH of the solution to pH 4.2. It's important to adjust the pH of the dilute ammonium hydroxide solution. If the initial pH is higher than pH 4.3, a little dilute HCL should be used to lower the pH, then adjust the pH back to a range of between pH 4.1 to pH 4.3. The ammonium ions are necessary for proper staining to occur.
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Can A5533 be utilized for fluorescent imaging, specifically for confocal imaging purposes?
1 answer-
The fluorescent properties of Alizarin Red S are not tested. As for its use in confocal microscopy, there are no applications mentioned.
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Hi, do you have a protocol for preparing the alizarin red s powder and for staining human osteoblast cells to detect mineralisation? Thank you
1 answer-
This product is not routinely tested for staining osteoblast cells. A general calcium staining protocol for use with paraffin-embedded samples is available below:
Calcium Staining protocol for use with Alizarin Red S
Fixation: 10 % Neutral Buffered Formalin or alcoholic formalin. Do not use calcium-containing fixatives
Embedding: Embed in paraffin and cut sections at 5 microns
Preparation of solutions:
Alizarin Red S, certified 2 grams
Distilled water 100 mL
Mix well and adjust the pH to 4.1 to 4.3 with a dilute ammonium hydroxide solution.
Procedure:
1. Deparaffinize, hydrate slides, and place slides in 50 % alcohol.
2. Rinse rapidly with distilled water.
3. Lay slides flat and cover with the alizarin red s solution.
4. Observe the reaction under a microscope and remove when a red-orange lake forms (usually 30 seconds to 5 minutes. The lake should be heavy, but not too diffused.
5. Shake off excess dye and blot, carefully.
6. Dehydrate in acetone for 10 to 20 seconds and in acetone-xylene (50:50) for 10 to 20 seconds.
7. Clear in xylene and mount in a synthetic mounting medium
Results:
Calcium deposits (except oxalate) will stain orange-red. Precipitate is birefringent.Alternatively, product TMS-008, Alizarin Red Staining Solution, has been tested for use with human and rodent osteocytes.
See the links below to review the product information, as well as an application note:
TMS-008 Alizarin-Red Staining Solution:
https://www.sigmaaldrich.com/product/mm/tms008Osteocyte Differentiation Application Note:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-osteogenesisHelpful?
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This product can be used in a in vivo bone immunolabeling ? I see a work available for you in this site using the Alizarin Red (A5533) to study bone regeneration. They inject the alizarin red S intraperitoneal some days before to sacrifice the animals.
1 answer-
This product has not been tested for in vivo or in vitro applications. Suitability would have to be determined by the end-user. Please see the link below to a publication that may be helpful:
https://pubmed.ncbi.nlm.nih.gov/15746986/Helpful?
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Can you please help clarify the difference between Alizarin-Red (TMS-008) and Alizarin Red S (A5533)? Also, in what situations would one be more suitable than the other?
1 answer-
Product A5533 is an Alizarin Red S powder while the TMS-008 is simply the 40 mM solution in water. Both products are used in the staining of cartilage and bone tissue. The A5533 is a Sigma-Aldrich brand and the TMS-008 is an EMD Millipore brand. Functionally, these products may be used for the same application.
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Is there an available excitation / emission specrtrum for this Alizarin Red S for fluorescence studies?
1 answer-
Various publications differ somewhat. Most sources report an excitation range of 530-560nm and the emission is expected to be 580nm. Significant shifts from these values can be found in the literature from due to varying conditions - pH, solvent, and sample type.
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I see that Product A5533, Alizarin Red S, was tested according to the Dahl method at pH 7.0. Can the dye also be used in the McGee-Russell procedure at pH 4.2?
1 answer-
Yes. The dye can be used at a variety of pH values.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the pH transition ranges for Product A5533, Alizarin Red S?
1 answer-
Although the pH transition ranges are not determined on a lot to lot basis, the solution changes from yellow to red in the range of 3.5-6.5, and from the orange to violet at pH 9.4-12. On a lot to lot basis there can be considerable difference in regards to color and pH ranges.
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