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43816

Sigma-Aldrich

DL-二硫代苏糖醇 溶液

BioUltra, for molecular biology, ~1 M in H2O

同義詞:

克莱兰氏试剂, (±)-二硫苏糖醇,消旋-二硫苏糖醇,二硫苏糖醇, (±)-苏式-1,4-二巯基-2,3-丁二醇 溶液, DTT

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About This Item

經驗公式(希爾表示法):
C4H10O2S2
CAS號碼:
分子量::
154.25
Beilstein:
1719757
MDL號碼:
分類程式碼代碼:
12352201
PubChem物質ID:
NACRES:
NA.25
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等級

for molecular biology

品質等級

產品線

BioUltra

化驗

900-1100 mmol/L (Titration molarity)

形狀

liquid

反應適用性

reagent type: reductant

濃度

~1 M in H2O

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值

2.7-5.0

密度

1.04 g/mL at 20 °C

吸收

cut-off at 330 nm

適合性

in accordance for filter test
suitable for molecular biology

儲存溫度

2-8°C

SMILES 字串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI 密鑰

VHJLVAABSRFDPM-QWWZWVQMSA-N

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一般說明

二硫苏糖醇(DTT)是巯基保护剂,[1] 它是一种防止蛋白质中巯基氧化的良好试剂。 DTT溶液是一种强效的多功能二硫化物还原剂。[2]它通过形成六元环并引发巯基-二硫键交换反应,使二硫键还原。

應用

DL-二硫苏糖醇溶液已被用于:

  • 处理肠外植体(intestinal explant)[3]
  • 制备酶分析用鳃细胞(gill cell)的匀浆液 [4]
  • 用于蛋白质印迹的蛋白质电泳[5]
  • 转谷氨酰胺酶活性[6]
  • SDS-PAGE用样品缓冲液[7]
  • 蛋白质印迹,作为单细胞核RNA测序用裂裂解缓冲液成分[8]
  • 作为细胞裂解用蛋白质提取缓冲液的成分[8]

生化/生理作用

DL-二硫苏糖醇(DTT)被用于化学脱粘技术,有助于去除生物膜中的细菌。[9]它可以通过破坏GABA(B1(a))受体结构中的四个半胱氨酸残基之间的二硫键,改变GABA(B)(γ-氨基丁酸)受体的稳定性。[10]二硫苏糖醇(DTT)广泛用于合成肽的合成和巯基蛋白质的生物化学重排。它也可用于研究蛋白质化学,如酶活性和蛋白质折叠。DTT控制巯基-二硫化物交换反应,从而完全消除了生物分子中的分子间或分子内亚原子二硫键。硫醇和DTT的环状二硫化物是该反应的产物。

特點和優勢

  • BioUltra 级- 1M DTT 溶液,适用于分子生物学
  • 不含DNA酶、RNA酶、磷酸酶和蛋白酶

其他說明

如需了解生化试剂系列的更多信息,请填写此表

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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存取文件庫

New Technologies, An Issue of Orthopedic Clinics (2018)
Expression and Localization of Cathepsins B, D, and G in Dupuytren?s Disease
Tan K, et al.
Plastic and reconstructive surgery. Global open, 6(2), e0133549-e0133549 (2018)
Suppression of GABA B receptor function in vivo by disulfide reducing agent, dl-dithiothreitol (DTT)
Carai M A M, et al.
Psychopharmacology, 174(2), 283-290 (2004)
Delivery of human immunodeficiency virus vaccine vectors to the intestine induces enhanced mucosal cellular immunity
Wang L, et al.
Journal of Virology, 83(14), 7166-7175 (2009)
In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring
Dib A, et al.
Frontiers in physiology, 9, 350-350 (2018)

文章

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

採用 mPAGE® TurboMix 技術的 Bis-Tris SDS-PAGE 凝膠澆注試劑盒,可快速、可靠地進行凝膠澆注和蛋白質分離。

條款

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. How stable is this DTT solution ? it is known that DTT is unstable in solution and should be kept under inert atmosphere. How long can I keep it once open ?

    1 answer
    1. This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.

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