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重要文件

H2899

Supelco

HPLC 蛋白标准混标

analytical standard

同義詞:

蛋白校准混标

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About This Item

分類程式碼代碼:
85151701
NACRES:
NA.24
暫時無法取得訂價和供貨情況

等級

analytical standard

品質等級

形狀

solid

分析物化學類別

amino acids, peptides, proteins

技術

HPLC: suitable

應用

food and beverages

形式

multi-component solution

儲存溫度

−20°C

應用

使用高效液相色谱法 (HPLC) 分离细菌细胞提取物样品中的蛋白质时,使用 HPLC 蛋白标准品混合物作为标准品。[1]

包裝

样品瓶,含核糖核酸酶 A、细胞色素 c、转铁蛋白、脱辅基肌红蛋白各约 1mg。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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Distribution, diversity, and activities of sulfur dioxygenases in heterotrophic bacteria
Liu H, et al.
Applied and Environmental Microbiology, 80, 1799-1806 (2014)
Honglei Liu et al.
Applied and environmental microbiology, 80(5), 1799-1806 (2014-01-07)
Sulfur oxidation by chemolithotrophic bacteria is well known; however, sulfur oxidation by heterotrophic bacteria is often ignored. Sulfur dioxygenases (SDOs) (EC 1.13.11.18) were originally found in the cell extracts of some chemolithotrophic bacteria as glutathione (GSH)-dependent sulfur dioxygenases. GSH spontaneously

Questions

1–4 of 4 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Could you provide an order of retention times of when each of these components elute off a protein C4 column (H2899 protein mixture)?  Thank you.

    1 answer
    1. The exact method is considered proprietary. However, for a C4 column, it should elute ribonuclease A first, followed by cytochrome C, holo-transferrin, and apomyoglobin.

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  4. What is each componant's actual molecular weight (ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin)?

    1 answer
    1. The molecular weight of each component is not reported. See below for the expected values:
      Ribonuclease A: approx. 13.7 kDa
      Cytochrome C: approx. 12.3 kDa
      holo-Transferrin: 72.1 - 78.5 kDa
      Myoglobin: approx. 17.6 kDa

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