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重要文件

H1115000

透明质酸酶

European Pharmacopoeia (EP) Reference Standard

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About This Item

CAS號碼:
EC號碼:
分類程式碼代碼:
41116107
NACRES:
NA.24
暫時無法取得訂價和供貨情況

等級

pharmaceutical primary standard

製造商/商標名

EDQM

應用

pharmaceutical (small molecule)

形式

neat

儲存溫度

2-8°C

SMILES 字串

O1COc2c1cc(cc2)C(CC(=O)c3cc4c(cc3)OCO4)C(=O)O

InChI

1S/C18H14O7/c19-13(11-2-4-15-17(6-11)25-9-23-15)7-12(18(20)21)10-1-3-14-16(5-10)24-8-22-14/h1-6,12H,7-9H2,(H,20,21)

InChI 密鑰

PPEBBOHQEAQKSW-UHFFFAOYSA-N

一般說明

本品按现行药典规定交付。所有本产品相关的支持信息,包括SDS和任何产品信息宣传册,均是在药典的授权下制定和发布的。
如需进一步信息和支持,请访问现行药典网站。

應用

透明质酸酶EP参考标准品的预期用途是欧洲药典规定的实验室测试。

包裝

本品按照现行药典要求提供。有关当前单位数量,请见EDQM 参考目录

其他說明

可能会销售限制。

相關產品

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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Sara Darbandi et al.
Turkish journal of urology, 45(3), 164-170 (2019-03-01)
Studies showed a decrease of the semen analysis parameters and an increase in the average age of first-time fathers over the past several decades. The aim of the present study was to assess the influence of paternal age on semen
Seyed Mojtaba Hosseini et al.
Asian journal of neurosurgery, 13(4), 1101-1107 (2018-11-22)
Spinal cord injury (SCI) as one of the most important diseases of central nervous system (CNS) without any definite treatment is still growing in incidence. In addition to trauma, some surgeries such as cardiac and thoracic aorta surgery may result
Erratum to: Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis.
Tae Jin Yun et al.
Methods in molecular biology (Clifton, N.J.), 1559, E1-E1 (2017-10-19)
Wei Xiang et al.
Nature communications, 9(1), 2574-2574 (2018-07-04)
Metabolic reprogramming greatly contributes to the regulation of macrophage activation. However, the mechanism of lipid accumulation and the corresponding function in tumor-associated macrophages (TAMs) remain unclear. With primary investigation in colon cancer and confirmation in other cancer models, here we
Junyan Sun et al.
Cell cycle (Georgetown, Tex.), 17(13), 1591-1601 (2018-06-19)
Psychological stress, which exerts detrimental effects on human reproduction, may compromise the meiotic competence of oocytes. Meiotic resumption, germinal vesicle breakdown (GVBD), is the first milestone to confer meiotic competence to oocytes. In the practice of assisted reproductive technology (ART)

Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. 1 mL 中にヒアルロニダーゼ活性が 120 単位になるよう、本品を正確に調製するしたいと考えております。具体的な手順をご教示いただけますと幸いです。

    1 answer
    1. This is a product of the European Pharmacopoiea. The current lot activity is reported in the product leaflet, available at the supplier product page.. The specific dilution procedure is described in the associated EP monograph or general chapter. Please see the link below to review the current leaflet:
      https://crs.edqm.eu/db/4DCGI/leaflet?leaflet=H1115000_3.pdf

      Helpful?

  4. Could you please tell me in which solvent this enzyme should be dissolved? Is it PBS or the culture medium?

    1 answer
    1. This is a product of the European Pharmacopoeia (EP) and is intended for use as an analytical standard. Please see the link below to visit the European Pharmacopeia website for more information and to download the product leaflet:
      https://crs.edqm.eu/db/4DCGI/View=H1115000

      The current shipping lot reports an activity of 577 IU/mg and a package size of 50 mg. For analytical purposes, the EP recommends dissolving the proper amount of hyaluronidase in hyaluronidase diluent. The latter consists of mixing 100 mL of pH 6.4 phosphate buffer solution with 100 mL of water and then dissolving 0.140 g of hydrolyzed gelatin in the solution at 37 °C. It is recommended to prepare fresh solutions.

      A similar research grade, H3506 Hyaluronidase from bovine testes, recommends dissolving the product at 1mg/mL in 0,02 M phosphate buffer, pH 7, with 77 mM sodium chloride and 0.01% BSA. For best results, prepare solutions fresh. Please see the link below to review the datasheet for this alternate product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdf

      Helpful?

  5. Hi, what is the activity of the hyaluronidase H1115000?

    1 answer
    1. The activity of hyaluronidase in Product No. H1115000 is lot-specific and is listed in the information leaflet hosted on the European Directorate for the Quality of Medicines & HealthCare (EDQM) website at https://crs.edqm.eu/db/4DCGI/View=H1115000.

      Helpful?

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