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H3757

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type VIII, lyophilized powder, 300-1,000 U/mg

同義詞:

玻璃糖醛酸酶, 玻璃酸酶

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About This Item

CAS號碼:
37326-33-3
酶委員會編號:
3.2.1.35 (BRENDA, IUBMB)
EC號碼:
253-464-3
MDL號碼:
MFCD00131351
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bovine testis

品質等級

200

共軛

conjugate (Glucosaminoglycan)

種類

Type VIII

形狀

lyophilized powder

比活性

300-1,000 U/mg

分子量

~55 kDa (four subunits of 14 kDa each)

UniProt登錄號

Q5E985
Q8SQG8

相關疾病

cancer

儲存溫度

−20°C

基因資訊

cow ... HYAL1(515397) , HYAL2(281838)

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相關類別

生化／生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

單位定義

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

準備報告

由无菌灌装的I-S型溶液制备。

其他說明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Kohtaro Miyazawa et al.
Histochemistry and cell biology, 133(1), 125-134 (2009-10-16)
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and
Chaemoon Lim et al.
Journal of cancer prevention, 27(2), 122-128 (2022-07-23)
Osteosarcoma is the most frequent primary malignant bone tumor with higher incidences in children and adolescents. Despite clinical evolutions, patients with osteosacoma have had a poor prognosis. There has been increasing evidence that cancer is a stem cell disease. This
Takayuki Yamochi et al.
Journal of assisted reproduction and genetics, 38(10), 2591-2599 (2021-05-11)
To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. When OGCs
Nathan V Welham et al.
Journal of speech, language, and hearing research : JSLHR, 52(4), 1008-1020 (2009-07-31)
To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, and vocal fold vibratory changes resulting from vocal fold scar. Twenty-four 4-month-old male Sprague-Dawley rats were assigned to 1 of 4 experimental groups: chronic vocal
C Rollo et al.
Reproduction (Cambridge, England), 154(4), 375-385 (2017-09-08)
Acetylation of histone proteins is a major determinant of chromatin structure and function. Fertilisation triggers a round of chromatin remodelling that prepares the genome for the first round of transcription from the new embryonic genome. In this study we confirm
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條款

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明質酸酶的酵素分析 (3.2.1.35)

為了測量透明質酸酶的活性，在 600 nm 波長下使用濁度測定法。當 pH 值為 5.35、溫度為 37 °C 時，一個單位的透明質酸酶活性每分鐘可導致 0.330 的吸光度變化。

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