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D9564

Sigma-Aldrich

DAPI,二乳酸

DAPI, dilactate
1 of 1 reviewers received a sample product or took part in a promotion

≥98% purity (HPLC), powder

同義詞:

4 & # 8242;,6-二脒基-2-苯吲哚,二乳酸酯

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About This Item

經驗公式(希爾表示法):
C16H15N5 · 2C3H6O3
分子量::
457.48
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
暫時無法取得訂價和供貨情況

產品名稱

DAPI,二乳酸, ≥98% (HPLC)

品質等級

化驗

≥98% (HPLC)

形狀

powder

技術

titration: suitable

顏色

yellow

溶解度

H2O: 20 mg/mL

&epsilon ;(消光係數)

28.00-32.50 at 345-351 nm in methanol

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-8°C

SMILES 字串

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI 密鑰

LCAUPANIXBMNIA-UHFFFAOYSA-N

一般說明

DAPI是一种吲哚林染料。它携带两种脒成分,一种在苯基环上,另一个直接在吲哚部分上。

應用

DAPI二乳酸盐已用于细胞的核染色。[1][2]DAPI是一种常用的流式细胞术染色剂,广泛应用于DNA和核染色。此外,它还用于原位杂交和其他免疫荧光程序后的复染剂。

生化/生理作用

一种用于检测单个核酸分子的高灵敏度染料。DAPI已经被证明能检测溶液中的单一的脱氧核糖核酸分子,并能检测单细胞和叶绿体中的脱氧核糖核酸图谱。

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Muller glial cells.
Chavira-Suarez E, et al.
Biochemical and Biophysical Research Communications, 404, 678-683 (2011)
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
Vigano F, et al.
Nature Neuroscience, 16, 1370-1372 (2013)
Silvia S Kang et al.
The Journal of experimental medicine, 208(4), 747-759 (2011-04-06)
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Richa K Dave et al.
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Hedgehog (Hh) signaling is crucial for the generation and maintenance of both embryonic and adult stem cells, thereby regulating development and tissue homeostasis. In the developing neocortex, Sonic Hedgehog (Shh) regulates neural progenitor cell proliferation. During neurogenesis, radial glial cells

Questions

1–4 of 4 Questions  
  1. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer
    1. One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm).

      Helpful?

  2. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer
    1. The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    1 answer
    1. As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. St. Louis, MO
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Good Quality

    Arrived quickly. Labeling is dimmer than expected.

    Helpful?

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