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重要文件

C7698

Sigma-Aldrich

环己酰亚胺,大包装

from microbial, ≥94% (TLC)

同義詞:

3- [2-(3,5-二甲基-2-氧代环己基)-2-羟乙基]戊二酰亚胺, 放线菌酮, 纳拉霉素A

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About This Item

經驗公式(希爾表示法):
C15H23NO4
CAS號碼:
分子量::
281.35
Beilstein:
88868
EC號碼:
MDL號碼:
分類程式碼代碼:
12352111
PubChem物質ID:
NACRES:
NA.77
暫時無法取得訂價和供貨情況

生物源

microbial

化驗

≥94% (TLC)

形狀

powder

顏色

white to off-white

溶解度

ethanol: soluble, clear to hazy

抗生素活性譜

fungi
yeast

作用方式

protein synthesis | interferes

儲存溫度

2-8°C

SMILES 字串

[H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

InChI 密鑰

YPHMISFOHDHNIV-FSZOTQKASA-N

基因資訊

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一般說明

环己酰亚胺,也称放线菌酮,是一种戊二酰亚胺娄抗生素,通常来源于灰色链霉菌。它可用作真核细胞中蛋白质生物合成的强效抑制剂,其机理是破坏翻译中的转运步骤,从而有效阻止翻译延伸(translational elongation)。在微生物学领域,作为酵母和真菌抗性菌株的选择剂它起到关键作用,在受控实验环境中非常重要。

在细胞生物学和生物化学研究领域,环己酰亚胺体现出与诱导细胞凋亡有关的双重特性,即根据细胞类型不同诱导或抑制细胞凋亡。快速且可逆的作用令其成为研究细胞过程和测定蛋白质半衰期的理想选择。环己酰亚胺也在生物医学研究中得到广泛应用,它抑制体外(生物体外)研究的真核细胞中的蛋白质合成,去除环己酰亚胺则可快速逆转其作用。这一特性使环己酰亚胺成为细胞生物学、生物医学和生物化学研究的最佳选择,为实验带来了精准的控制和多功能性。

應用

  • 在酵母菌株中,环己酰亚胺已被用作环己酰亚胺追踪实验中的蛋白质合成抑制剂。[1]
  • 它已被用于抑制哺乳动物细胞中的翻译。[2][3]
  • 它已被用于抑制真菌生长。[4]

生化/生理作用

作用机制:真核生物中的翻译抑制导致细胞生长停滞和细胞死亡。CHX被广泛用于抗CHX的酵母和真菌菌株的选择、蛋白质合成的受控抑制以检测短寿命蛋白质和蛋白质表达的超诱导作用、以及诱导凋亡或促进通过死亡受体诱导的凋亡。

活性谱:具有抑制酵母和真菌如念珠菌、曲霉、酵母菌和青霉的活性

特點和優勢

  • 适用于多种研究应用的优质抗生素
  • 常用于细胞生物学和生物化学研究

儲存和穩定性

密闭。干燥。于通风良好处保存。上锁保存,或保存在仅对有资格或授权人员
开放的区域。

其他說明

保存于密闭容器内,置于干燥通风处。置于干燥处保存。
如需了解生化试剂系列的更多信息,请填写此表

訊號詞

Danger

危險分類

Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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