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Merck
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F6057

Sigma-Aldrich

Fluoroshield 封固剂(含DAPI)

liquid

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About This Item

分類程式碼代碼:
12171500
NACRES:
NA.47

產品名稱

Fluoroshield 封固剂(含DAPI), histology mounting medium

品質等級

形狀

liquid

成份

Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl

技術

immunofluorescence: suitable

pH值範圍

7.9-8.3

溶解度

water: soluble

螢光

λex 360 nm; λem 460 nm

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-8°C

一般說明

含DAPI的Fluoroshield是一种含有4′,6-二氨基-2-苯基吲哚(DAPI)的水固载介质,也是一种核反染剂,可用作蓝色荧光DNA探针。它是一种抗褪色剂,用于保存组织和细胞涂片的荧光,通过防止快速光漂白和最小化眨眼介导的现象。(1)用于原位杂交技术或其他需要DNA染色荧光的方法。DAPI的激发波长为360nm,发射波长为460nm,产生蓝色荧光。RNA也可以采用DAPI染色。

應用

Fluoroshield封固剂(含DAPI)可用于 :
  • 用作免疫细胞化学中的复染剂,可标记神经祖细胞的细胞核
  • 用作免疫荧光研究中心脏组织部分的组织学封固剂
  • 用作细胞核检测中免疫组化染色心脏部分的封固溶液

法律資訊

FluoroShield is a trademark of ImmunoBioScience Corp.

相關產品

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker
Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
Maria L Spletter et al.
eLife, 7 (2018-05-31)
Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group

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包含β-肌动蛋白、α-微管蛋白和波形蛋白用于细胞骨架结果蛋白活细胞分析的高滴度慢病毒颗粒。

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