推薦產品
生物源
bacterial (Arthrobacter ureafaciens)
品質等級
形狀
solution
比活性
~25 units/mg protein
包裝
pkg of 1 U (100 μl)
製造商／商標名
Roche
最適pH
5.0-5.5
儲存溫度
2-8°C
一般說明
神经氨酸酶可水解通过α2,3-、α2,6-或α2,8-连接至寡糖、多糖、粘多糖、糖蛋白和糖脂的N-或O-酰基神经氨酸末端（水解率：α2,6 > α2,3 > α2,8）。需要注意的是，水解糖脂需要存在表面活性剂。由于具有广泛的底物特异性，该酶非常适合从各种生物材料的糖缀合物中完全去除唾液酸。[1]
特異性
可切割与Gal、GlcNAc、GalNAc、AcNeu、GlcNeu、寡糖、糖脂或糖蛋白连接的α2,3-、α2,6-或α2,8-末端唾液酸残基。其切割的相对速率为α2,6 >α2,3 >α2,8，该结果在三糖和四糖中的化学键上进行测定。
應用
对于糖脂的水解，需要表面活性剂的存在。由于具有广泛的底物特异性，该酶非常适合从各种生物材料的糖缀合物中完全去除唾液酸。
物理性質
该产品是具有以下分子量的同工酶（L，M1，M2和S）的混合物： ~52 kDa、66 kDa和88 kDa。
外觀
溶于10 mM磷酸钠、0.1％Micr-O-Protect（w/v）、0.25 mg/ml牛血清白蛋白(pH 7)溶液中
準備報告
工作浓度：酶/底物比率应在0.04U/25-80 μg范围内。
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
水污染物質分類（WGK）
nwg
閃點（°F）
does not flash
閃點（°C）
does not flash
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Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
神經氨酸酶可用於裂解蛋白質中的鍵氨酸。在本方案中，將來自霍亂弧菌的酵素用於固定細胞上。
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