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重要文件

S7705

Sigma-Aldrich

TRAPeze1X CHAPS裂解缓冲液

1X CHAPS Lysis Buffer is a component of the gel-and ELISA-based TRAPeze Telomerase Detection Kits & is used in the extraction step of the procedure.

同義詞:

CHAPS Lysis Buffer

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About This Item

分類程式碼代碼:
41116012
eCl@ss:
32160405
NACRES:
NA.32
暫時無法取得訂價和供貨情況

品質等級

製造商/商標名

Chemicon®

運輸包裝

wet ice

一般說明

1X CHAPS 裂解缓冲液是基于凝胶和 ELISA 的 TRAPeze 端粒酶检测试剂盒的组件,用于该过程的提取步骤。
用作 TRAPEZE 试剂盒检测法的补充试剂,当较大组织样品需要使用额外的 1X CHAPS 裂解缓冲液才能实现有效的细胞裂解即可使用该试剂。 它通过使该试剂盒能分析较大的组织样本,从而扩展了试剂盒的功能。

應用

TRAPeze1X CHAPS裂解缓冲液已用于:
  • 用端粒酶定量活性测定(Q-TRAP)提取HEK293细胞总蛋白[1]
  • 搅匀小鼠心房组织以进行蛋白质印迹分析[2]
  • 在端粒酶表达分析细胞系中重悬细胞以确定蛋白浓度[3]

儲存和穩定性

储存于 4°C。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TRAPEZE is a trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Sigridur K Bodvarsdottir et al.
Mutation research, 729(1-2), 90-99 (2011-10-25)
In the present study the possible involvement of telomeres in chromosomal instability of breast tumors and cell lines from BRCA2 mutation carriers was examined. Breast tumors from BRCA2 mutation carriers showed significantly higher frequency of chromosome end-to-end fusions (CEFs) than
Yu-Feng Hu et al.
Biochimica et biophysica acta. Molecular basis of disease, 1867(5), 166088-166088 (2021-01-31)
Point mutation in alcohol dehydrogenase 2 (ALDH2), ALDH2*2 results in decreased catalytic enzyme activity and has been found to be associated with different human pathologies. Whether ALDH2*2 would induce cardiac remodeling and increase the attack of atrial fibrillation (AF) remains
Manuel Bernabé-García et al.
Molecular oncology, 15(7), 1818-1834 (2021-03-14)
Telomerase reverse transcriptase (TERT) maintains telomere homeostasis, thus ensuring chromosome stability and cell proliferation. In addition, several telomere-independent functions of human TERT have been described. In this study, we report that TERT binds directly to the TCF binding elements located

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