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重要文件

C9426

Sigma-Aldrich

CHAPS水合物

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

同義詞:

3-[3-(胆酰胺丙基)二甲氨基]丙磺酸 水合物, CHAPS

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About This Item

經驗公式(希爾表示法):
C32H58N2O7S · xH2O
CAS號碼:
分子量::
614.88 (anhydrous basis)
MDL號碼:
分類程式碼代碼:
12161902
PubChem物質ID:
NACRES:
NB.22
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描述

zwitterionic

產品線

BioReagent

化驗

≥98% (HPLC)

形狀

powder

分子量

614.88 g/mol (anhydrous basis)

技術

electrophoresis: suitable
protein quantification: suitable

雜質

<0.04% DMF

mp

157 °C (dec.) (lit.)

溶解度

water: 50 mg/mL, clear, colorless

適合性

suitable for electrophoresis

儲存溫度

room temp

SMILES 字串

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI 密鑰

SJCUTFKCLFLIFE-JWTJKVBLSA-N

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一般說明

CHAPS是一种非变性两性离子磺基甜菜碱洗涤剂。它温和,可溶解膜蛋白和受体。它有助于打破蛋白质-蛋白质相互作用。它是天然存在的胆汁盐的衍生物。[1]其胶束分子量较小(6,150)、临界胶束浓度很高(6-10 mM),因此可通过透析从样品中去除。

應用

CHAPS的用途:
  • 用于细胞膜透化[2]
  • 作为裂解缓冲液中的组分,用于肝组织和脂肪组织均质化[3][4]
  • 作为酶表征变性缓冲液的组分[5]
  • 作为2D电泳中2D-样品缓冲液的组分[6]
  • 用于等电聚焦中的蛋白溶解[7]

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Acute Tox. 4 Oral

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How do I store CHAPS, once it's dissolved in H2O?

    1 answer
    1. Solutions prepared at 100 mM in water are stable for at least 1 year when stored refrigerated. Note that a prepared solution is available. Please see the link below to review:
      https://www.sigmaaldrich.com/product/sigma/19899

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