535142
Protease Inhibitor Cocktail I
Animal-Free, lyophilized, for the inhibition of serine, esterases, cysteine, trypsin-like and metalloproteases. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
同義詞:
Animal-Free Protease Inhibitor Mix, Protease inhibitor
登入查看組織和合約定價
全部照片(1)
About This Item
分類程式碼代碼:
12352200
NACRES:
NA.77
暫時無法取得訂價和供貨情況
推薦產品
產品名稱
Protease Inhibitor Cocktail Set I, Animal-Free, The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
形狀
lyophilized
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
desiccated (hygroscopic)
溶解度
water: soluble
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H2O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.
應用
Protease Inhibitor Cocktail Set I, Animal-Free has been used:
- as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines[1]
- in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting[2]
- as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell[3]
生化／生理作用
Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.
警告
Toxicity: Standard Handling (A)
重構
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
標靶器官
Respiratory Tract
儲存類別代碼
8A - Combustible corrosive hazardous materials
水污染物質分類（WGK）
WGK 3
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Ca2+/Calmodulin-dependent protein kinase II inhibits hepatitis B virus replication from cccDNA via AMPK activation and AKT/mTOR suppression
Kim J, et al.
Microorganisms, 10(3), 498-498 (2022)
Rongxuan Zhu et al.
Frontiers in oncology, 12, 848483-848483 (2022-04-26)
Rapid tumor growth inevitably results in energy stress, including deficiency of glutamine, a critical amino acid for tumor cell proliferation. However, whether glutamine deficiency allows tumor cells to use lipid droplets as an energy resource and the mechanism underlying this
Waqas Azeem et al.
Frontiers in immunology, 11, 438-438 (2020-04-16)
The transcription factor β-catenin is able to induce tolerogenic/anti-inflammatory features in different types of dendritic cells (DCs). Monocyte-derived dendritic cells (moDCs) have been widely used in dendritic cell-based cancer therapy, but so far with limited clinical efficacy. We wanted to
Detection of HBV C Protein Phosphorylation in the Cell
Jung J and Kim K
Bio-protocol, 5(15), e1551-e1551 (2015)
Yan Yan et al.
Cancer communications (London, England), 40(12), 694-710 (2020-10-06)
Dopamine and dopamine receptor D1 (DRD1), a member of the dopamine receptor family, have been indicated to play important roles in cancer progression, but dopamine secretion in hepatocellular carcinoma (HCC) and the effects of DRD1 on HCC remain unclear. This
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
