The optimal pH range for product P8375 is 6.0 - 6.5.
推薦產品
種類
Type VI
形狀
essentially salt-free, lyophilized powder
比活性
≥250 units/mg solid (using pyrogallol)
分子量
~44 kDa
溶解度
0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
吸光率
RZ 2.5-4.0
應用
diagnostic assay manufacturing
儲存溫度
2-8°C
SMILES 字串
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI 密鑰
JSPLKZUTYZBBKA-UHFFFAOYSA-N
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一般說明
辣根过氧化物酶是从辣根（Amoracia rusticana）中分离出来的，属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成，具体取决于特定同工酶。其分子量（约44 kDa）包括多肽链（33,890道尔顿）、氯化血红素加Ca2+ （约700道尔顿）和碳水化合物（约9,400道尔顿）。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。
應用
辣根过氧化物酶（HRP）分离自辣根（紫穗槐），属于过氧化物酶的铁原卟啉基团。 它被用作western blot、ELISA和免疫组化等生化应用。 辣根过氧化物酶用于扩增弱信号并提高靶标分子如蛋白的可检测性。 产品P8375，VI型，是一种基本不含盐的冻干粉。 它常用于测定溶液中葡萄糖和过氧化物的量。 它已用于研究颈脊髓神经元的感觉输入[3]。
生化／生理作用
HRP易与过氧化氢（H2O2）结合，而所得的[HRP-H2O2]络合物可氧化各种氢供体。最适pH为6.0-6.5而酶在5.0-9.0的pH范围内最为稳定。HRP可以通过几种不同的方法与抗体结合，包括戊二醛，高碘酸盐氧化，通过二硫键，以及通过氨基和巯基定向交联剂。它比酶标记β-半乳糖苷酶和碱性磷酸酶更小且更稳定，因此是最理想的标记。此外，它的糖基化会导致更低低的非特异性结合。[4]
当与底物一起孵育时，辣根过氧化物酶可产生标记分子的有色、荧光或发光衍生物，从而可实现定量。 辣根过氧化物酶已被证明可略微降低cydAB突变体中的抑制水平。 已知的抑制剂包括叠氮化钠、氰化物、L-胱氨酸、重铬酸盐、亚乙基硫脲、羟胺、硫化物、钒酸盐、对氨基苯甲酸以及Cd2+、Co2+、Cu2+、Fe3+、Mn2+、Ni2+以及Pb2+离子。
單位定義
一个连苯三酚单元将在20℃、pH 6.0条件下，在20秒内从连苯三酚形成1.0mg的红棓酚。
分析報告
RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度，而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。
其他說明
有关过氧化物酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
基底
產品號碼
描述
訂價
抑制劑
產品號碼
描述
訂價
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
探索我們的辣根過氧化酶、產品、底物和抑制劑，滿足您的 ELISA、免疫分析和蛋白質應用需求。
條款
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
建立以 2,2'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）为基质的过氧化物酶测定方法。
相關內容
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
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What is the optimal pH range of Peroxidase from horseradish, Product P8375?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What can you tell me about the stability of Peroxidase from horseradish, Product P8375, (as is, in suspension and in solution)?
1 answer-
This product is stable as a powder, kept refrigerated. See the certificate of analysis for a lot-specific recommended retest date. It is also stable as a crystalline suspension of 3.2 M (NH4)2SO4 solution containing potassium phosphate buffer, pH 6.0 (see Product No. P6140). It is reasonably stable in solution in phosphate buffer. We have found that at 10 mg/mL in 0.1 phosphate buffer, pH 6.0, solutions kept frozen at -20°C lose less than 2% of their activity per week.
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How do I convert units of Peroxidase from horseradish, Product P8375, to solid?
1 answer-
The activity listed on the Certificate of Analysis for each lot can be used to convert from units to milligrams of solid.
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