跳轉至內容
Merck
全部照片(2)

文件

P2088

Sigma-Aldrich

过氧化物酶 来源于辣根

Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

同義詞:

供体:过氧化氢氧化还原酶, 辣根过氧化物酶

登入查看組織和合約定價


About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

horseradish

形狀

essentially salt-free, lyophilized powder

比活性

200-300 units/mg solid (using pyrogallol)

分子量

~44 kDa

吸光率

RZ 2.6-3.4

儲存溫度

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI 密鑰

JSPLKZUTYZBBKA-UHFFFAOYSA-N

尋找類似的產品? 前往 產品比較指南

相關類別

一般說明

辣根过氧化物酶是从辣根(Amoracia rusticana)中分离出来的,属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成,具体取决于特定同工酶。其分子量(约44 kDa)包括多肽链(33,890道尔顿)、氯化血红素加Ca2+ (约700道尔顿)和碳水化合物(约9,400道尔顿)。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。

應用

辣根过氧化物酶已被用于脱氢聚合物 (DHP) 的研究,以研究单寡聚物偶联机制。 它还被用来通过创建辣根过氧化物酶模拟DNAzyme来帮助开发用于microRNA(miRNA)检测的比色方法。

生化/生理作用

已证明辣根过氧化物酶可催化多种物质的聚合,包括对乙酰氨基酚和腰果酚。

注意

在低 pH 值和较高温度下保持活性。

單位定義

一个连苯三酚单元将在20℃、pH 6.0条件下,在20秒内从连苯三酚形成1.0mg的红棓酚。

準備報告

通过伯胺的化学保护而保持稳定。

分析報告

RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度,而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。

其他說明

有关过氧化物酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

D W Potter et al.
Molecular pharmacology, 29(2), 155-162 (1986-02-01)
Horseradish peroxidase catalyzed the polymerization of acetaminophen. Addition of reduced glutathione (GSH) to reaction mixtures resulted in decreased polymerization and formation of minor amounts of GSH-acetaminophen conjugates. The conjugates were identified as 3-(glutathion-S-yl)acetaminophen and 3-(glutathion-S-yl)diacetaminophen. Horseradish peroxidase also catalyzed polymerization
Yanqin Wen et al.
Analytical chemistry, 84(18), 7664-7669 (2012-08-30)
We present a highly sensitive colorimetric method for microRNA (miRNA) detection. This method is based on a rolling-circle amplification (RCA) DNA machine, which integrates RCA, nicking enzyme signal amplification and DNAzyme signal amplification. The DNA machine is triggered by the
Keehoon Won et al.
Biomacromolecules, 5(1), 1-4 (2004-01-13)
Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at
Sun-Joo Moon et al.
Phytochemistry, 82, 15-21 (2012-08-14)
In this study, dehydrogenative polymers (DHP) were synthesized in vitro through dehydrogenative polymerization using different ratios of coniferyl alcohol (CA) and sinapyl alcohol (SA) (10:0, 8:2, 6:4, 2:8, 0:10), in order to investigate the monolignol coupling mechanism in the presence
Jane M Natividad et al.
PloS one, 4(7), e6472-e6472 (2009-08-04)
Excessive uptake of commensal bacterial antigens through a permeable intestinal barrier may influence host responses to specific antigen in a genetically predisposed host. The aim of this study was to investigate whether intestinal barrier dysfunction induced by indomethacin treatment affects

文章

Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.

探索我們的辣根過氧化酶、產品、底物和抑制劑,滿足您的 ELISA、免疫分析和蛋白質應用需求。

條款

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

建立以 2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)为基质的过氧化物酶测定方法。

查看全部

相關內容

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務