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R4642

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

别名:

RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶

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10 MG
$53.50
50 MG
$176.00
250 MG
$545.00
1 G
$1,330.00

$53.50

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10 MG
$53.50
50 MG
$176.00
250 MG
$545.00
1 G
$1,330.00

About This Item

CAS号:
9001-99-4
EC 号:
3.1.27.5 (BRENDA, IUBMB)
MDL號碼:
MFCD00132181
分類程式碼代碼:
12352204
NACRES:
NA.53

$53.50

请联系客服了解存货情况
新价格，新优惠！

生物源

bovine pancreas

品質等級

200

等級

for molecular biology

形狀

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

分子量

13.7 kDa
 ~13,700

濃度

20-40 mg/mL

適合性

suitable for

異物活動

Endonuclease and exonuclease, none detected
NICKase and DNase, none detected

儲存溫度

−20°C

SMILES 字串

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI 密鑰

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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一般說明

RNA 酶 A 是一种内切核糖核酸酶，攻击嘧啶核苷酸的 3'OH磷酸。切割 pG-pG-pC-pA-pG 的序列，得到 pG-pG-pCp 和 A-pG。单链 RNA 表现出最高的活性。
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。

應用

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。
适用于：
  • RNA 酶保护实验
  • 去除非特异性结合的 RNA
  • RNA 序列分析
  • 水解蛋白质样品中含有的 RNA
  • 质粒 DNA 纯化

特點和優勢

我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。

成分

RNA 酶 A 以含有 10mM Tris-HCl 的 50％ 甘油的溶液（pH8.0）形式提供。

單位定義

RNA 酶 A 的一个主要应用是从质粒 DNA 制品中去除 RNA。对于该应用，使用不含 DNA 酶的 RNA 酶 A，终浓度为 10ug/mL。

该 RNase A 产物的原液不需要灭活残留的 DNA 酶，可能导致 RNA 酶沉淀和酶活性丧失。如果 RNA 酶 A 溶液在中性 pH 下加热，则会发生沉淀。当在较低 pH 下加热时，由于存在蛋白质杂质，可能发生一些沉淀。

分析報告

蛋白测定方法：E.

其他說明

RNA 酶 A 的激活剂包括钾盐和钠盐。 RNA 酶 A 可被 His12 或 His119 的烷基化抑制。

應用

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抑制劑

产品编号
说明
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脱氧核糖核酸酶 I 无 RNase 溶液 来源于牛胰腺

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核糖核酸酶A 来源于牛胰腺, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

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蛋白酶 来源于灰色链霉菌, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

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象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000399885
0000381928
0000381929
0000381925
0000381924

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Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine
Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the
Alessandro Ori et al.
Genome biology, 17, 47-47 (2016-03-16)
Recent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at
R Perdigão-Henriques et al.
Oncogene, 35(2), 158-172 (2015-03-24)
The miR-200 family promotes the epithelial state by suppressing the Zeb1/Zeb2 epithelial gene transcriptional repressors. To identify other miR-200-regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells. In all, 520 mRNAs were significantly enriched
Netta Mäkinen et al.
PLoS genetics, 12(2), e1005850-e1005850 (2016-02-20)
Uterine leiomyosarcomas (ULMSs) are aggressive smooth muscle tumors associated with poor clinical outcome. Despite previous cytogenetic and molecular studies, their molecular background has remained elusive. To examine somatic variation in ULMS, we performed exome sequencing on 19 tumors. Altogether, 43
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核糖核酸酶A(EC 3.1.27.5)的酶促测定

本实验方案可用于测定核糖核酸酶A(RNase A)的活性。

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

相关内容

HPLC Analysis of Proteins on TSKgel® BioAssist S

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Chromatograms

application for HPLC

Questions

1–6 of 6 Questions  

  1. What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?

    1 answer

    1. The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation

      Please see the link below to review additional information is the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdf

      Helpful?

  2. Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?

    1 answer

    1. The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.

      Helpful?

  3. Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution.  What does the solution contain?

    1 answer

    1. The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?

    1 answer

    1. This product is stable for at least 2 years when stored properly at -20 °C.

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  6. When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?

    1 answer

    1. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.

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