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Merck
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文件

R4875

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺

Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

别名:

RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶

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About This Item

CAS号:
9001-99-4
EC 号:
3.1.27.5 (BRENDA, IUBMB)
EC號碼:
232-646-6
MDL號碼:
MFCD00132181
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bovine pancreas

品質等級

300

種類

Type I-A

形狀

powder

比活性

≥50 Kunitz units/mg protein

分子量

~13,700

濃度

≥60% (RNase A, SDS-PAGE)

技術

cell based assay: suitable

雜質

salt, essentially free

適合性

suitable for molecular biology

應用

diagnostic assay manufacturing

異物活動

protease, essentially free

儲存溫度

−20°C

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI 密鑰

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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一般說明

RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。

應用

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。

生化／生理作用

核糖核酸酶A是一种内切核糖核酸酶，可在嘧啶核苷酸后切割单链RNA。 它在3'磷酸基末端进行攻击。 核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。 RNase A可被His12和His119的烷基化所抑制并被钾盐和钠盐所活化。

特點和優勢

我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。

準備報告

盐分级和色谱纯化。

分析報告

蛋白测定方法：E.

應用

产品编号
说明
价格

抑制劑

产品编号
说明
价格

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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Yang Han et al.
eLife, 7 (2018-08-25)
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise
Elizaveta Katorcha et al.
PLoS pathogens, 14(6), e1007093-e1007093 (2018-06-22)
The main risk of emergence of prion diseases in humans is associated with a cross-species transmission of prions of zoonotic origin. Prion transmission between species is regulated by a species barrier. Successful cross-species transmission is often accompanied by strain adaptation
D Joseph-McCarthy et al.
Protein engineering, 9(9), 773-780 (1996-09-01)
One relatively new computational approach to the drug discovery process involves calculating functional group maps of a target structure. Experimental functional group mapping techniques have also recently emerged. In this paper, the structure of RNase A with two bound formates
Franziska Büscheck et al.
World journal of urology, 39(3), 829-837 (2020-05-04)
DNA ploidy measurement has earlier been suggested as a potentially powerful prognostic tool in many cancer types, but the role in renal tumors is still unclear. To clarify its prognostic impact, we analyzed the DNA content of 1320 kidney tumors
Barbara Schöpf et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(6), 1895-1900 (2012-01-11)
Single strand nicks and gaps in DNA have been reported to increase the efficiency of nucleosome loading mediated by chromatin assembly factor 1 (CAF-1). However, on mismatch-containing substrates, these strand discontinuities are utilized by the mismatch repair (MMR) system as
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实验方案

核糖核酸酶A(EC 3.1.27.5)的酶促测定

本实验方案可用于测定核糖核酸酶A(RNase A)的活性。

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

相关内容

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Chromatograms

application for HPLC

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