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Merck
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文件

D5758

Sigma-Aldrich

焦碳酸二乙酯

96% (NT)

别名:

DEP, DEPC, 氧二甲酸二乙酯, 焦炭酸二乙酯, 重碳酸二乙酯

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About This Item

线性分子式:
O(COOC2H5)2
CAS号:
1609-47-8
分子量:
162.14
Beilstein:
637031
EC號碼:
216-542-8
MDL號碼:
MFCD00009106
分類程式碼代碼:
12352202
PubChem物質ID:
24893964
NACRES:
NA.77

生物源

synthetic (microbial fermentation/ cell culture)

化驗

96% (NT)

形狀

liquid

折射率

n20/D 1.398 (lit.)

bp

93-94 °C/18 mmHg (lit.)

密度

1.101 g/mL at 25 °C (lit.)

運輸包裝

wet ice

儲存溫度

2-8°C

SMILES 字串

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI 密鑰

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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一般說明

焦碳酸二乙酯（DEPC）是一种用于灭活RNase酶并对水分和pH敏感的化学制品。在155℃时它在水溶液中可分解成乙醇和二氧化碳。DEPC对能将其分解成氨基甲酸乙酯这种潜在致癌物的氨也很敏感。

應用

焦碳酸二乙酯已在PCR反应中用于处理去离子水，以降低了RNA被RNase降解的风险。它还可以用于斑点印迹杂交，以稀释分离自不同微生物的总RNA。
蛋白中His及Tyr残基的修饰试剂。用于dsDNA结构破坏的稳健探针，可与完全或部分未堆叠的碱基发生反应。

生化／生理作用

DEPC是一种潜在的核酸酶抑制剂。0.1%的DEPC溶液用于灭活RNase。
它可以剂量和时间依赖的方式抑制兰尼碱与骨骼肌中兰尼碱/ Ca2+受体通道的结合，并提高SR囊泡的Ca2+通透性。
在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H302

危險分類

Acute Tox. 4 Oral

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

156.2 °F - closed cup

閃點（°C）

69 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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H Perry-O'Keefe et al.
Journal of applied microbiology, 90(2), 180-189 (2001-02-13)
A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of
Properties of mouse globin messenger ribonucleic acid and its preparation in milligram quantities.
R Williamson et al.
Biochemistry, 10(16), 3014-3021 (1971-08-03)
V Shoshan-Barmatz et al.
The Biochemical journal, 299 ( Pt 1), 177-181 (1994-04-01)
Exposure of junctional sarcoplasmic reticulum (SR) membranes or purified ryanodine receptor to the histidine-specific reagent diethyl pyrocarbonate (DEPC) led to concentration- and time-dependent inactivation of ryanodine binding. The pH-dependence of the inactivation of ryanodine binding by DEPC and the reversal
E C Williamson et al.
Journal of clinical microbiology, 39(1), 47-50 (2001-01-04)
Respiratory tract colonization with Scedosporium apiospermum in patients with chronic suppurative lung disease is a significant concern for lung transplantation candidates, since Scedosporium infections occurring posttransplantation are usually untreatable. Up to 10% of patients with cystic fibrosis attending our respiratory
Melina Papalampropoulou-Tsiridou et al.
Frontiers in cellular neuroscience, 14, 103-103 (2020-06-09)
Injury and inflammation cause tissue acidosis, which is a common feature of various painful conditions. Acid-Sensing Ion channels (ASICs) are amongst the main excitatory channels activated by extracellular protons and expressed in the nervous system. Six transcripts of ASIC subunits
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商品

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

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