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Merck

10109134001

Roche

核糖核酸酶

from bovine pancreas

别名:

RNA酶

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500 MG
$296.00

$296.00


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500 MG
$296.00

About This Item

UNSPSC代码:
12352204

$296.00


请联系客服了解存货情况

生物来源

bovine pancreas

质量水平

表单

dry powder (prepared from crystallized ribonuclease)

比活

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

包装

pkg of 500 mg

制造商/商品名称

Roche

运输

wet ice

储存温度

2-8°C

一般描述

人类染色体 14q11.2 中显示存在所有人类 rna 酶。其中只有 8 种 (RNase 1-8) 被真正称为典型的 rna 酶。剩下的 9 到 13 个被称为非典型 rna 酶。人类核糖核酸酶 1 被称为 HP-RNase 或人类胰腺核糖核酸酶。人类核糖核酸酶 2 也被称为 EDN(嗜酸性粒细胞衍生的神经毒素),是从嗜酸性粒细胞分离出来的。[1]
核糖核酸酶的粗混合物。干粉(由结晶核糖核酸酶制备)。

特异性

热灭活: 1 mM EDTA

应用

RNase 已被用于琼脂糖凝胶电泳分析 DNA 片段。[2]已在流式细胞术分析期间使用。[3]
核糖核酸酶 (RNase) 已用于 DNA 梯形分析。[4]

单位定义

一个单位是在测定条件下 1 分钟内引起 A0 至 A1 吸光度降低的酶活性;A 0 至 A1 对应于总转化率,A1 为最终吸光度。

制备说明

活化剂: PK 需要 Mg2+(或 Mn2+、Co2+)和 K+(或 NH4+、Rb+)才能发挥全部活性。
工作液:建议将 RNase (1 mg/mL) 溶于水或 TE 缓冲液 (pH 7.5) 中。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ribonucleases (2011)
S Saito et al.
The Journal of biological chemistry, 275(44), 34528-34533 (2000-08-10)
Apoptosis of cardiac myocytes is one of the causes of heart failure. Here we examine the mechanism by which the activation of beta-adrenergic receptor induces cardiomyocyte apoptosis. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling and DNA ladder analyses revealed that
Ognjen Sekulović et al.
Methods in molecular biology (Clifton, N.J.), 1476, 143-165 (2016-08-11)
Bacteriophages (phages) are present in almost, if not all ecosystems. Some of these bacterial viruses are present as latent "prophages," either integrated within the chromosome of their host, or as episomal DNAs. Since prophages are ubiquitous throughout the bacterial world
Youmna S Kfoury et al.
Cell stem cell, 28(12), 2090-2103 (2021-09-23)
Extracellular vesicles (EVs) transfer complex biologic material between cells. However, the role of this process in vivo is poorly defined. Here, we demonstrate that osteoblastic cells in the bone marrow (BM) niche elaborate extracellular vesicles that are taken up by hematopoietic
David Olmeda et al.
Molecular biology of the cell, 14(7), 2844-2860 (2003-07-15)
Beta-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. Beta-catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems beta-catenin also acts as

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