价格与库存信息目前不能提供
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生物来源
human
质量水平
方案
>98% (SDS-PAGE)
表单
liquid
比活
≥1.3 x 10e6 units/mg protein
分子量
Mw 38,389 Da
浓度
0.5 mg/mL
技术
cell culture | stem cell: suitable
杂质
<0.05 ng/μg cytokine (LAL test)
输入
sample type induced pluripotent stem cell(s)
溶解性
water: soluble
适用性
suitable for molecular biology
运输
dry ice
一般描述
Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.
应用
外形
Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.
储存及稳定性
For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.
Further dilutions should be made into appropriate buffer or medium.
分析说明
The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Irene L Llorente et al.
Stem cell research, 55, 102458-102458 (2021-07-19)
White matter stroke (WMS) occurs as small infarcts in deep penetrating blood vessels in the brain and affects the regions of the brain that carry connections, termed the subcortical white matter. WMS progresses over years and has devastating clinical consequences.
Isaac Hurtado-Guerrero et al.
Journal of clinical medicine, 9(4) (2020-04-05)
Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß) receptor is generated through alternative splicing of IFNAR2 and
Roni A Hazim et al.
Stem cell research & therapy, 8(1), 217-217 (2017-10-04)
Dysfunction of the retinal pigment epithelium (RPE) is implicated in numerous forms of retinal degeneration. The readily accessible environment of the eye makes it particularly suitable for the transplantation of RPE cells, which can now be derived from autologous induced
商品
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
实验方案
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
产生人诱导多能干细胞（iPSC）的干细胞重编程实验方案，包括基于病毒和非病毒RNA的方法。
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