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P4707

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

Poly-L-lysine solution

synthetic, liquid, 0.01%, suitable for cell culture

别名:

PLL 溶液

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50 ML
$255.00

$255.00

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50 ML
$255.00

About This Item

CAS号:
25988-63-0
MDL號碼:
MFCD00165424
分類程式碼代碼:
12352202
eCl@ss:
32160406
NACRES:
NA.75

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产品名称

多聚-L-赖氨酸 溶液, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

無菌

sterile-filtered

品質等級

300

產品線

BioReagent

形狀

liquid

分子量

70—150 kDa

包裝

pkg of 50 mL

濃度

0.01%

技術

cell culture | mammalian: suitable

表面覆盖率

4 μg/cm2

雜質

endotoxin, tested

溶解度

water: soluble

運輸包裝

ambient

儲存溫度

2-8°C

SMILES 字串

N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI 密鑰

WBSCNDJQPKSPII-KKUMJFAQSA-N

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相关类别

一般說明

聚-L-赖氨酸是一种带正电荷的氨基酸聚合物，每个赖氨酸残基约有一个HBr。氢溴酸盐可使聚-L-赖氨酸以可溶于水的结晶形式存在。在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

應用

  • 聚-L-赖氨酸涂覆的玻璃盖玻片已用于对用于扫描电子显微镜的样本进行封片。[1]
  • 聚-L-赖氨酸涂覆的孔用于在电极上对PC-12细胞进行捕获。[2]
  • 聚-L-赖氨酸涂覆的硼硅酸盐玻片用于接种病毒载体转导的细胞。[3]
聚-L-赖氨酸聚合物可用于促进细胞对固体底物的粘附、与甲氨蝶呤结合以增加药物转运、胰岛微囊化、细胞微囊化技术、微阵列玻片涂层和染色体制剂。 较低分子量的聚-L-赖氨酸（30000-70000）在溶液中粘度较低，但分子量较高的聚赖氨酸在每个分子中提供更多的附着位点。

生化／生理作用

聚-L-赖氨酸是一种非特异性的细胞附着因子，通过增强细胞膜负离子与培养基表面的静电相互作用，促进细胞对固体基质的粘附。 当其吸附到细胞培养表面时，聚-L-赖氨酸的作用是增加可用于细胞结合的带正电荷位点的数量。 对于可降解聚-L-赖氨酸的细胞，应采用聚-D-赖氨酸作为附着因子。

成分

聚-L-赖氨酸是一种带正电荷的氨基酸聚合物，每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-L-赖氨酸以可溶于水的结晶形式存在。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

準備報告

该产品是以溶于水的0.01%无菌过滤溶液形式提供的。用该溶液进行涂覆的玻片应当随后进行一步5分钟的孵育并在室温对其进行干燥。 涂覆的玻片在免受灰尘的保护下可稳定一年。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0098
RNBL7086
RNBL4935
RNBL4203
RNBK9931

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实验方案

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

多聚-L-赖氨酸细胞附着实验方案

使用多聚-赖氨酸将细胞粘附在固相基质上，多聚-赖氨酸可增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用。

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Questions

1–10 of 12 Questions  

  1. When coating coverslips with item P4707, is it necessary to wash the reagent off after 5 minutes, or is there a different recommended procedure?

    1 answer

    1. The product is provided as a 0.01% sterile filtered solution in water. When coating slides with this solution, it is recommended to incubate for 5 minutes and allow the slides to dry at room temperature.

      Helpful?

  2. Is the product P4707 suitable for coating glassware for cell culture? Also, what is the difference between Poly L and Poly D lysine?

    1 answer

    1. Yes, the product P4707 is suitable for coating glassware for cell culture. The difference between Poly L and Poly D lysine lies in their stereoisomeric properties. Poly-L-Lysine is a charge enhancer and can be used for coating many surfaces, including tissue culture plasticware and glassware. However, certain cells can digest poly-L-lysine, in which case the poly-D-lysine, such as P7405, should be used. This product can also be used to coat tissue culture plasticware and glassware.

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  3. How many times can it be reused?

    1 answer

    1. The number of times that this product may be re-applied has not been determined. This would be dependent on several factors, such as surface area, material type, surface pretreatment, and incubation period. Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdf

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  4. What is the molecular weight of product P4707?

    1 answer

    1. Poly-L-lysine solution is 70-150 kDa molecular weight.

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  5. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?

    1 answer

    1. A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.

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  6. How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?

    1 answer

    1. Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.

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  7. Should I use Poly-L-Lysine or Poly-D-Lysine?

    1 answer

    1. Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.

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  8. What is the concentration of Product P4707, Poly-L-Lysine solution?

    1 answer

    1. This product is supplied as a 0.01% w/v solution.

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  9. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?

    1 answer

    1. For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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