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UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length

UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length application for UHPLC

Materiały

standardowe

Numer produktu
Opis
Cennik

Oxazepam solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Nordiazepam solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Diazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Temazepam solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Titan C18, 2 cm x 2.1 mm I.D., 1.9 μm particles (577120-U)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)

flow rate

various

temp.

35 °C

detector

TIC MRM (UV also runs in series)

injection

1 μL

sample

100 ng/mL in mobile phase

Opis

Komentarz do analizy

These two sets of chromatograms show separations on 100, 50, 30, and 20 mm x 2.1 mm I.D. columns and at different flow rates and demonstrate the utility of the 1.9 μm Titan particles. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.

Informacje prawne

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