T7924

Transforming Growth Factor-α human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonim(y): hTGF-α, TGF-α
• Wzór empiryczny (zapis Hilla): C₂₃₉H₃₄₈N₇₀O₇₂S₆
• Numer CAS: 105186-99-0
• Masa cząsteczkowa: 5546.14
• Numer MDL: MFCD00082077
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Nazwa produktu: Transforming Growth Factor-α human, TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
• pochodzenie biologiczne: human
• Poziom jakości: 100
• rekombinowane: expressed in E. coli
• Próba: ≥98% (SDS-PAGE and HPLC)
• Formularz: powder
• siła działania: ≤0.2 ng/mL ED₅₀
• jakość: endotoxin tested
• masa cząsteczkowa: 5.5 kDa
• opakowanie: pkg of 1 mg; pkg of 5 mg
• warunki przechowywania: avoid repeated freeze/thaw cycles
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: ≤1 EU/μg
• numer dostępu UniProt: P01135
• temp. przechowywania: −20°C
• informacje o genach: human ... TGFA(7039)

Opis

Amino Acid Sequence

Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]

Opis ogólny

The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33.[1] TNFα is a member of TNF superfamily.[2]

Zastosowanie

Transforming Growth Factor-α human has been used:

• to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles[3]
• in human hepatocytes cell culture and keratinocyte cell culture[4][5]
• to induce morphogenesis in primary mouse organoids[2]

Działania biochem./fizjol.

The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses.[6] Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy.[7] The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.[6]

Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.

Postać fizyczna

Lyophilized from a 0.2 μm filtered buffered solution.

Komentarz do analizy

The biological activity is measured by its ability to stimulate ³H-thymidine incorpation into BALB/c 3T3 cells.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)