SRP4693

Apolipoprotein A−I human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonim(y): APOA1, Apo-AI, Apolipoprotein A-I, C117399, MGC117399
• Numer CAS: 1426185-50-3
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in E. coli
• Próba: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Formularz: lyophilized
• masa cząsteczkowa: ~28 kDa
• opakowanie: pkg of 100 μg
• warunki przechowywania: avoid repeated freeze/thaw cycles
• zanieczyszczenia: endotoxin, tested
• numer dostępu NCBI: NM_000039
• numer dostępu UniProt: P02647
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• informacje o genach: human ... ApoA1(335)

Opis

Opis ogólny

ApoA-I (apolipoprotein A-I) is a 29.0kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle.[1][2] Recombinant human ApoA-I is a 28.2kDa protein of 244 amino acid residues.

ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Zastosowanie

Apolipoprotein A-I human has been used in the cholesterol efflux assay.[3][4]

Działania biochem./fizjol.

ApoA-I (apolipoprotein A-I), which is found exclusively in HDL (high-density lipoprotein), has a unique ability to capture and solubilize free cholesterol.[5] This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion.[6] This process, called reverse cholesterol transport, is thought to inhibit atherogenesis.[7] For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.[8] Changes in the level of serum apoA-I may serve as a prognostic marker for non-metastatic nasopharyngeal carcinoma.[9] Low levels of apoA-I in the plasma is linked to hyperhomocysteinemia.[10]

Postać fizyczna

Sterile filtered and Lyophilized without additives.

Uwaga dotycząca przygotowania

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Rekonstytucja

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4 °C for 1 week or –20 °C for future use.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable