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Dokumenty

SRP3096

Sigma-Aldrich

Interleukin-6 human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Synonim(y):

Interleukin-6 human, IL-6

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About This Item

Numer MDL:
MFCD00131403
Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

human

Poziom jakości

300

rekombinowane

expressed in E. coli

Próba

≥95% (SDS-PAGE)

Postać

lyophilized

siła działania

≤2.0 ng/mL ED50

masa cząsteczkowa

20.9 kDa

opakowanie

pkg of 100 μg
pkg of 20 μg

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤1 EU/μg endotoxin, tested

numer dostępu UniProt

P05231

Warunki transportu

wet ice

temp. przechowywania

-10 to -25°C

informacje o genach

human ... IL6(3569)

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Opis ogólny

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.

Zastosowanie

IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).

Działania biochem./fizjol.

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Opakowanie

100 μg
20 μg

Sekwencja

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Postać fizyczna

Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers.

Rekonstytucja

Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum).

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H319,H335

Klasyfikacja zagrożeń

Eye Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Activation of STAT3 stimulates AHSP expression in K562 cells.
Cao C, et al.
Science China: Life Sciences, 57, 488-494 (2014)
Common variants in genes that mediate immunity and risk of multiple myeloma.
Brown EE, et al.
International Journal of Cancer. Journal International Du Cancer, 120, 2715-2722 (2007)
Nur Mahammad et al.
Molecules (Basel, Switzerland), 26(24) (2021-12-25)
Cytosolic phospholipase A2α (cPLA2α) is the rate-limiting enzyme in releasing arachidonic acid and biosynthesis of its derivative eicosanoids. Thus, the catalytic activity of cPLA2α plays an important role in cellular metabolism in healthy as well as cancer cells. There is
Nikolai Litviakov et al.
Oncotarget, 11(21), 1988-2001 (2020-06-12)
The phenomenon of non-CSC (cancer stem cell) to CSC plasticity has been previously described in multiple studies and occurs during the ectopic expression of stemness genes such as OCT3, SOX2, KLF4, MYC, NOTCH1, and NANOG. In our opinion, acquiring the
Olga Pershina et al.
Biomedicines, 9(9) (2021-09-29)
New drug targets, markers of disease prognosis, and more efficient treatment options are an unmet clinical need in breast cancer (BC). We have conducted a pilot study including patients with luminal B stage breast cancer IIA-IIIB. The presence and frequency
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Przeprowadzanie testów kolorymetrycznych w celu nieradioaktywnej kwantyfikacji proliferacji, żywotności i cytotoksyczności komórek przylegających lub zawiesinowych hodowanych w 96-dołkowych mikropłytkach.

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