SRP3008

APRIL human

recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonim(y): A Proliferating-inducing Ligand, TNFSF13, TRDL-1?
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: lyophilized
• Assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in Hi-5 Insect cells
• Mol wt: 16.3 kDa

Właściwości

• biological source: human
• recombinant: expressed in Hi-5 Insect cells
• assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: 16.3 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: O75888
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TNFSF13(8741)

Opis

General description

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues. 11

Biochem/physiol Actions

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.

Physical form

Lyophilized from 20 mM Sodium Acetate, pH 7.5 + 500 mM NaCl.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable