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SRE0055

Sigma-Aldrich

Protease Inhibitor Cocktail Powder

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

Synonim(y):

Protease Inhibitor Cocktail powder

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

pochodzenie biologiczne

bovine lung
yeast

Formularz

lyophilized powder

temp. przechowywania

−20°C

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Specyficzność

Inhibits serine, cysteine, and metallo-proteases.

Zastosowanie

Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.

Działania biochem./fizjol.

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.

Komponenty

1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Ilość

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Uwaga dotycząca przygotowania

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
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Piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Odwiedź Bibliotekę dokumentów

Farhan Younas et al.
Biochimica et biophysica acta. Biomembranes, 1860(8), 1544-1553 (2018-05-23)
Legionella pneumophila is an aerobic and nonspore-forming pathogenic Gram-negative bacterium of the genus Legionella. It is the causative agent of Legionnaires' disease, also known as Legionellosis. The hosts of this organism are diverse, ranging from simple water borne protozoans such
Amina E Essawy et al.
PeerJ, 9, e11930-e11930 (2021-08-27)
Bisphenol A (BPA) is an endocrine disruptor to which humans are often subjected during daily life. This study aimed to investigate the ameliorative effect of astragaloside IV (ASIV) or saponins extracted from Astragalus spinosus (A. spinosus) against DNA damage and
Xianzhang Zeng et al.
Microbiome, 11(1), 204-204 (2023-09-12)
Aging is a significant risk factor for ischemic stroke and worsens its outcome. However, the mechanisms for this worsened neurological outcome with aging are not clearly defined. Old C57BL/6J male mice (18 to 20 months old) had a poorer neurological
Biology for the IB Diploma: Standard and Higher Level (2001)
Ahmed S Al-Shami et al.
Molecular and cellular biochemistry (2024-06-28)
Bisphenol A (BPA), a common endocrine-disrupting chemical, is found in a wide range of home plastics. Early-life BPA exposure has been linked to neurodevelopmental disorders; however, the link between neuroinflammation, pyroptosis, and the development of psychiatric disorders is rarely studied.

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