PPC1010 does not contain EDTA
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PPC1010
Protease and Phosphatase Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase
Synonim(y):
Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1170,00 zł | |
| 5 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2480,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
1170,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócNazwa produktu
Protease and Phosphatase Inhibitor Cocktail,
form
DMSO solution
Quality Level
packaging
pkg of 1 ml, 5 ml ×
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
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1 of 1
Ta pozycja | |||
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
| packaging pkg of 1 ml, 5 ml × | packaging - | packaging - | packaging - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in - |
General description
Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.
Application
Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
- for cellular protein extraction
- to lyse purified CD4+ T cells
- to prepare protein extracts from placental tissues and cells
Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.
Other Notes
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Klasa składowania
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
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Powiązane treści
Metody lizy komórek i ekstrakcji białek obejmują przegląd różnych technik, od rozpuszczania detergentami po mechaniczne rozbijanie, wspierając potrzeby badawcze.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Instructions
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| PPC1010-5ML | 04061835538416 |
| PPC1010-1ML | 04061835538409 |
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Does PPC1010 contain EDTA?
1 answer-
Helpful?
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Does these Protease and Phosphatase Inhibitor Cocktail contains any type of metal chelator or EDTA?
1 answer-
This product does not contains EDTA or other chelators. See the link below to review the product datasheet which includes the cocktail components:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdfHelpful?
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