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P2714

Sigma-Aldrich

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

Synonim(y):

Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.77
Formularz:
lyophilized powder
temp. przechowywania:
−20°C

Nazwa produktu

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

pochodzenie biologiczne

bovine lung

Poziom jakości

300

Formularz

lyophilized powder

temp. przechowywania

−20°C

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Opis ogólny

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Specyficzność

Inhibits serine, cysteine, and metallo-proteases.

Zastosowanie

Protease Inhibitor Cocktail powder has been used as a component:
  • in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
  • in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
  • in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
  • in 0.1 M NaCl solution to wash and collect skin secretions of frog

Działania biochem./fizjol.

The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Komponenty

This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Przestroga

The lyophilized powder is stable for 4 years when stored at -20°C.

Ilość

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Uwaga dotycząca przygotowania

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Inne uwagi

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
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Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315 - H319

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000308021
0000233200
0000159074
068M4099V
069M4108V

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
Veterninary Research, 49(1), 1-6 (2018)
Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
F Maier et al.
Osteoarthritis and cartilage, 27(9), 1382-1391 (2019-05-24)
Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
Tens of millions suffer from insulin deficiency (ID); a defect leading to severe metabolic imbalance and death. The only means for management of ID is insulin therapy; yet, this approach is sub-optimal and causes life-threatening hypoglycemia. Hence, ID represents a
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Produkty

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Powiązane treści

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Wybór inhibitora proteazy: Unikanie utraty białek podczas przygotowywania próbek

Wybierz różne typy inhibitorów proteaz w zależności od potrzeb, aby zapobiec degradacji białek podczas izolacji i charakteryzacji oraz zabezpieczyć białka podczas przygotowywania próbek.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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