SAB5801018

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonim(y): Recombinant Alpaca single variable domain secondary Nanobody
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: alpaca
• rekombinowane: expressed in E. coli
• białko sprzężone: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• forma przeciwciała: affinity purified immunoglobulin
• rodzaj przeciwciała: secondary antibodies
• klon: recombinant monoclonal
• Postać: lyophilized solid
• masa cząsteczkowa: 14 kDa
• reaktywność gatunkowa: Alpaca, mouse
• metody: ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• rozmiar: 0.1 mL
• kolor: colorless
• izotyp: VHH
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgM(µ chain) single domain Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specyficzność

Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Opakowanie

100 μg in Glass bottle

Postać fizyczna

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Rekonstytucja

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Przechowywanie i stabilność

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Inne uwagi

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H317,H412
• Zwroty wskazujące środki ostrożności: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Klasyfikacja zagrożeń: Aquatic Chronic 3 - Skin Sens. 1
• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable